Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4326 | 5' | -57.1 | NC_001720.1 | + | 12405 | 0.66 | 0.515123 |
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Target: 5'- cGGGCUGgCGGCC-GGgGCCGguGUg -3' miRNA: 3'- cCUCGACaGCUGGaUCgUGGCguCGa -5' |
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| 4326 | 5' | -57.1 | NC_001720.1 | + | 24509 | 0.67 | 0.47346 |
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Target: 5'- --cGCUGUCGACggagagcguuuCUGGCGgaucagccguguCCGCGGCg -3' miRNA: 3'- ccuCGACAGCUG-----------GAUCGU------------GGCGUCGa -5' |
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| 4326 | 5' | -57.1 | NC_001720.1 | + | 32459 | 0.68 | 0.432587 |
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Target: 5'- aGGAGCUGUCagugGugCUguagaGGCuaacgauGCUGCAGCa -3' miRNA: 3'- -CCUCGACAG----CugGA-----UCG-------UGGCGUCGa -5' |
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| 4326 | 5' | -57.1 | NC_001720.1 | + | 1199 | 0.68 | 0.414355 |
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Target: 5'- cGGAGCUGUCGGaacuGa--CGCAGCUa -3' miRNA: 3'- -CCUCGACAGCUggauCgugGCGUCGA- -5' |
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| 4326 | 5' | -57.1 | NC_001720.1 | + | 7194 | 0.69 | 0.368714 |
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Target: 5'- -aGGCUGUCGguaucGCCauagaGGCugCGCGGCUc -3' miRNA: 3'- ccUCGACAGC-----UGGa----UCGugGCGUCGA- -5' |
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| 4326 | 5' | -57.1 | NC_001720.1 | + | 6480 | 1.09 | 0.000427 |
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Target: 5'- cGGAGCUGUCGACCUAGCACCGCAGCUc -3' miRNA: 3'- -CCUCGACAGCUGGAUCGUGGCGUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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