Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4328 | 5' | -45.3 | NC_001720.1 | + | 7226 | 1.11 | 0.005597 |
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Target: 5'- gAAACAGGCUAUCAUCGCAUGAAAAGCc -3' miRNA: 3'- -UUUGUCCGAUAGUAGCGUACUUUUCG- -5' |
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| 4328 | 5' | -45.3 | NC_001720.1 | + | 7189 | 0.78 | 0.553461 |
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Target: 5'- cGAACAGGCUGUCGguaUCGCcauAGAGGCu -3' miRNA: 3'- -UUUGUCCGAUAGU---AGCGuacUUUUCG- -5' |
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| 4328 | 5' | -45.3 | NC_001720.1 | + | 2382 | 0.69 | 0.950139 |
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Target: 5'- -cACAGGCUAUCGcguacugcucuccccUCGUA--GGGAGCg -3' miRNA: 3'- uuUGUCCGAUAGU---------------AGCGUacUUUUCG- -5' |
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| 4328 | 5' | -45.3 | NC_001720.1 | + | 32024 | 0.69 | 0.954959 |
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Target: 5'- cAGCAGGCUAcCAuuccuaauugcggugUCGCGcuuggGGGAGGCg -3' miRNA: 3'- uUUGUCCGAUaGU---------------AGCGUa----CUUUUCG- -5' |
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| 4328 | 5' | -45.3 | NC_001720.1 | + | 6364 | 0.68 | 0.969666 |
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Target: 5'- --cUAGGCUGuagaacUCGUCGUAggagGGGAAGUg -3' miRNA: 3'- uuuGUCCGAU------AGUAGCGUa---CUUUUCG- -5' |
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| 4328 | 5' | -45.3 | NC_001720.1 | + | 32242 | 0.68 | 0.969666 |
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Target: 5'- -cACGGGUUAccuuugCAUCGUAUGggGuuaucGGCu -3' miRNA: 3'- uuUGUCCGAUa-----GUAGCGUACuuU-----UCG- -5' |
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| 4328 | 5' | -45.3 | NC_001720.1 | + | 4509 | 0.68 | 0.97273 |
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Target: 5'- gGAACAGaGCauuccuuUGUUcUCGCAUaGGAGAGCg -3' miRNA: 3'- -UUUGUC-CG-------AUAGuAGCGUA-CUUUUCG- -5' |
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| 4328 | 5' | -45.3 | NC_001720.1 | + | 25666 | 0.66 | 0.991072 |
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Target: 5'- ---gAGGCacaaaGUCGcuUgGCAUGAAAGGCg -3' miRNA: 3'- uuugUCCGa----UAGU--AgCGUACUUUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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