Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4332 | 5' | -55 | NC_001720.1 | + | 17352 | 0.68 | 0.509555 |
Target: 5'- cGCUugCGCCGCCuUCCGCCggUa----- -3' miRNA: 3'- -CGA--GCGGCGGuAGGCGGuuGauucuc -5' |
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4332 | 5' | -55 | NC_001720.1 | + | 29859 | 0.69 | 0.478003 |
Target: 5'- cCUcCGCCGCUGUUCGCUAACgc-GAGc -3' miRNA: 3'- cGA-GCGGCGGUAGGCGGUUGauuCUC- -5' |
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4332 | 5' | -55 | NC_001720.1 | + | 26514 | 0.7 | 0.399041 |
Target: 5'- cCUCGCCGuCCGccucggaaCCGCCGGCUccGAGc -3' miRNA: 3'- cGAGCGGC-GGUa-------GGCGGUUGAuuCUC- -5' |
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4332 | 5' | -55 | NC_001720.1 | + | 31028 | 0.71 | 0.371653 |
Target: 5'- cGCUCGCCGUCA-CagcaGCCGGCgUAGGGu -3' miRNA: 3'- -CGAGCGGCGGUaGg---CGGUUG-AUUCUc -5' |
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4332 | 5' | -55 | NC_001720.1 | + | 24872 | 0.75 | 0.211316 |
Target: 5'- uGCUCGCCGCgCAUUuuUGCCcACUGcAGAGa -3' miRNA: 3'- -CGAGCGGCG-GUAG--GCGGuUGAU-UCUC- -5' |
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4332 | 5' | -55 | NC_001720.1 | + | 11544 | 1.1 | 0.000655 |
Target: 5'- uGCUCGCCGCCAUCCGCCAACUAAGAGu -3' miRNA: 3'- -CGAGCGGCGGUAGGCGGUUGAUUCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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