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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 4342 | 3' | -55 | NC_001720.1 | + | 31285 | 0.66 | 0.653674 |
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Target: 5'- aCCAUGGGUacgCGucccaCGGgcGCGGUa -3' miRNA: 3'- aGGUACCCGaa-GCug---GCCauUGCCAg -5' |
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| 4342 | 3' | -55 | NC_001720.1 | + | 12397 | 0.66 | 0.675944 |
|
Target: 5'- gUCgCGUgcGGGCUggCGGCCGGggcCGGUg -3' miRNA: 3'- -AG-GUA--CCCGAa-GCUGGCCauuGCCAg -5' |
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| 4342 | 3' | -55 | NC_001720.1 | + | 37939 | 0.67 | 0.620141 |
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Target: 5'- gUCGggGGGCUUUGA-UGGUggUGGUCc -3' miRNA: 3'- aGGUa-CCCGAAGCUgGCCAuuGCCAG- -5' |
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| 4342 | 3' | -55 | NC_001720.1 | + | 10570 | 0.7 | 0.458562 |
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Target: 5'- cCCAcaGGGUUUCGAauaUGGUGGCGGg- -3' miRNA: 3'- aGGUa-CCCGAAGCUg--GCCAUUGCCag -5' |
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| 4342 | 3' | -55 | NC_001720.1 | + | 11136 | 0.74 | 0.249446 |
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Target: 5'- cUCUA-GGGCgcaGGCCGGU-GCGGUCg -3' miRNA: 3'- -AGGUaCCCGaagCUGGCCAuUGCCAG- -5' |
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| 4342 | 3' | -55 | NC_001720.1 | + | 21581 | 1.11 | 0.000599 |
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Target: 5'- aUCCAUGGGCUUCGACCGGUAACGGUCg -3' miRNA: 3'- -AGGUACCCGAAGCUGGCCAUUGCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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