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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 4344 | 3' | -53.1 | NC_001720.1 | + | 17276 | 0.67 | 0.684491 |
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Target: 5'- uCGCGGggGUGCcggACGAGGcgGcugcGCGAGc -3' miRNA: 3'- uGCGCCa-CACGa--UGUUCCuaC----UGCUC- -5' |
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| 4344 | 3' | -53.1 | NC_001720.1 | + | 22525 | 0.89 | 0.030397 |
|
Target: 5'- aACGCGGUGUGCUACAAGGAUcAgGAGc -3' miRNA: 3'- -UGCGCCACACGAUGUUCCUAcUgCUC- -5' |
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| 4344 | 3' | -53.1 | NC_001720.1 | + | 28020 | 0.72 | 0.403055 |
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Target: 5'- cGCGCGG-GUGacgaGCGAGGgcGGCGAGc -3' miRNA: 3'- -UGCGCCaCACga--UGUUCCuaCUGCUC- -5' |
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| 4344 | 3' | -53.1 | NC_001720.1 | + | 29446 | 0.66 | 0.761314 |
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Target: 5'- -gGCGG-GUGC-GCAAGGcuUGACGGu -3' miRNA: 3'- ugCGCCaCACGaUGUUCCu-ACUGCUc -5' |
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| 4344 | 3' | -53.1 | NC_001720.1 | + | 32004 | 0.74 | 0.299445 |
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Target: 5'- uUGCGGUGUcgcGCUugGGGGA-GGCGGGu -3' miRNA: 3'- uGCGCCACA---CGAugUUCCUaCUGCUC- -5' |
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| 4344 | 3' | -53.1 | NC_001720.1 | + | 35436 | 0.67 | 0.684491 |
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Target: 5'- gAUGCGGauaGUGUUAUcucuAGGGAUGACGcGGa -3' miRNA: 3'- -UGCGCCa--CACGAUG----UUCCUACUGC-UC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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