Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4385 | 5' | -55.9 | NC_001720.1 | + | 10135 | 0.66 | 0.634393 |
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Target: 5'- uGCCccGGAagcucuugaaGUACuuUCGGGUCUCUUCCa -3' miRNA: 3'- cCGGucCCU----------UAUG--AGCCUAGGGGAGG- -5' |
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| 4385 | 5' | -55.9 | NC_001720.1 | + | 20776 | 0.67 | 0.557648 |
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Target: 5'- cGCCAGcgacgaGGAAUcCcCGGAggCCCCUCUg -3' miRNA: 3'- cCGGUC------CCUUAuGaGCCUa-GGGGAGG- -5' |
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| 4385 | 5' | -55.9 | NC_001720.1 | + | 31126 | 0.67 | 0.557648 |
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Target: 5'- aGCCGGcGuGAAagccgGCUCGGA-CCCCUUa -3' miRNA: 3'- cCGGUC-C-CUUa----UGAGCCUaGGGGAGg -5' |
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| 4385 | 5' | -55.9 | NC_001720.1 | + | 36116 | 0.68 | 0.535067 |
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Target: 5'- uGGCCAGGaGAAUggguagaAUUaauaaGGAcUCCgCCUCCa -3' miRNA: 3'- -CCGGUCC-CUUA-------UGAg----CCU-AGG-GGAGG- -5' |
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| 4385 | 5' | -55.9 | NC_001720.1 | + | 14450 | 0.71 | 0.36869 |
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Target: 5'- uGGUgAGGGGuccGCUCGGGUCCguguugaCCUCg -3' miRNA: 3'- -CCGgUCCCUua-UGAGCCUAGG-------GGAGg -5' |
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| 4385 | 5' | -55.9 | NC_001720.1 | + | 19410 | 0.74 | 0.219615 |
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Target: 5'- cGGCCGGGGccacgagggcgucccGCUCGaGAUCCUCUUCa -3' miRNA: 3'- -CCGGUCCCuua------------UGAGC-CUAGGGGAGG- -5' |
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| 4385 | 5' | -55.9 | NC_001720.1 | + | 37712 | 1.13 | 0.000401 |
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Target: 5'- uGGCCAGGGAAUACUCGGAUCCCCUCCc -3' miRNA: 3'- -CCGGUCCCUUAUGAGCCUAGGGGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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