Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5118 | 3' | -55 | NC_001798.1 | + | 2364 | 0.65 | 0.954963 |
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Target: 5'- gUGAGcUCGGccaCGGC-CCGCGGGGCg- -3' miRNA: 3'- aGCUC-AGCUa--GCUGaGGUGCCUCGac -5' |
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| 5118 | 3' | -55 | NC_001798.1 | + | 105706 | 0.66 | 0.937646 |
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Target: 5'- aCGGGggcgacagCGGUCGACacCCA-GGGGCUGu -3' miRNA: 3'- aGCUCa-------GCUAGCUGa-GGUgCCUCGAC- -5' |
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| 5118 | 3' | -55 | NC_001798.1 | + | 42640 | 0.66 | 0.937646 |
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Target: 5'- ---cGUgGGUCGugUCCAUGGGGUc- -3' miRNA: 3'- agcuCAgCUAGCugAGGUGCCUCGac -5' |
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| 5118 | 3' | -55 | NC_001798.1 | + | 22497 | 0.67 | 0.910706 |
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Target: 5'- aCGGGgCGAUCGuccaCACGGAGCg- -3' miRNA: 3'- aGCUCaGCUAGCugagGUGCCUCGac -5' |
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| 5118 | 3' | -55 | NC_001798.1 | + | 47648 | 0.67 | 0.90461 |
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Target: 5'- -aGAGUcCGcgCGACgacuuccgCCGCGGcGCUGg -3' miRNA: 3'- agCUCA-GCuaGCUGa-------GGUGCCuCGAC- -5' |
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| 5118 | 3' | -55 | NC_001798.1 | + | 69377 | 0.68 | 0.898281 |
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Target: 5'- cUCGGGUagcgcgcguuccCGGagaCGACUCCGCGGGGUc- -3' miRNA: 3'- -AGCUCA------------GCUa--GCUGAGGUGCCUCGac -5' |
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| 5118 | 3' | -55 | NC_001798.1 | + | 136012 | 0.68 | 0.891723 |
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Target: 5'- -gGAGUCGGUCGGCguccCCGUGGAuCUGu -3' miRNA: 3'- agCUCAGCUAGCUGa---GGUGCCUcGAC- -5' |
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| 5118 | 3' | -55 | NC_001798.1 | + | 15581 | 0.72 | 0.691227 |
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Target: 5'- gCGGGgaguggCGA-CGACUCCACGGAuGCa- -3' miRNA: 3'- aGCUCa-----GCUaGCUGAGGUGCCU-CGac -5' |
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| 5118 | 3' | -55 | NC_001798.1 | + | 141703 | 0.97 | 0.025315 |
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Target: 5'- gUCGAGUCGAUCGA-UCCACGGAGCUGg -3' miRNA: 3'- -AGCUCAGCUAGCUgAGGUGCCUCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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