miRNA: 3'- -UCCUCUUa--Ga--------GCGCCUUGUUGu-AGca -5'
miRNA: 3'- -UCCUCUUa---GAGCGCCU--------------UGUUgUAGCa -5'
miRNA: 3'- -UCCUCUUAGa------GCGCCUUGUugUa-GCa -5'
miRNA: 3'- -UCCUCUUAGAGCGCCUUGUUGUAGCA- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5122 | 5' | -50 | NC_001798.1 | + | 35137 | 0.66 | 0.996313 |
|
Target: 5'- gAGGAGAAgggCgcgccggugCGCGGAGCAGCcuUCc- -3' miRNA: 3'- -UCCUCUUa--Ga--------GCGCCUUGUUGu-AGca -5' |
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| 5122 | 5' | -50 | NC_001798.1 | + | 134611 | 0.66 | 0.99613 |
|
Target: 5'- aAGGAGGGcgccUUCGCGGAccuggucgagguguGCGAgGUCGg -3' miRNA: 3'- -UCCUCUUa---GAGCGCCU--------------UGUUgUAGCa -5' |
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| 5122 | 5' | -50 | NC_001798.1 | + | 151825 | 0.66 | 0.995674 |
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Target: 5'- gGGGGGGAUUuagagggCGCGGGGCGugAggCGg -3' miRNA: 3'- -UCCUCUUAGa------GCGCCUUGUugUa-GCa -5' |
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| 5122 | 5' | -50 | NC_001798.1 | + | 139229 | 1.08 | 0.013513 |
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Target: 5'- gAGGAGAAUCUCGCGGAACAACAUCGUc -3' miRNA: 3'- -UCCUCUUAGAGCGCCUUGUUGUAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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