miRNA: 3'- caGGGGCGUCGCGAauuucauuaGGa -5'
miRNA: 3'- cAGGGGCGU-CGCGAa---UUUCAUUaGGA- -5'
miRNA: 3'- -CAGGGGCGuCGCGaauuucaUUAGGa -5'
miRNA: 3'- -CAGGGGCGUCGCGAAUUUCAUUAGGA- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5126 | 5' | -51.3 | NC_001798.1 | + | 25214 | 0.67 | 0.975177 |
|
Target: 5'- -gCCCCGCGGCGCUgacccg---CCg -3' miRNA: 3'- caGGGGCGUCGCGAauuucauuaGGa -5' |
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| 5126 | 5' | -51.3 | NC_001798.1 | + | 41944 | 0.68 | 0.968161 |
|
Target: 5'- aUUCCCGCGcGUGCUcgggAAAGUGAgCCUc -3' miRNA: 3'- cAGGGGCGU-CGCGAa---UUUCAUUaGGA- -5' |
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| 5126 | 5' | -51.3 | NC_001798.1 | + | 105769 | 0.68 | 0.968161 |
|
Target: 5'- aGUCCCCGC-GCGCccgcg--GGUCCg -3' miRNA: 3'- -CAGGGGCGuCGCGaauuucaUUAGGa -5' |
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| 5126 | 5' | -51.3 | NC_001798.1 | + | 137066 | 1.07 | 0.009152 |
|
Target: 5'- gGUCCCCGCAGCGCUUAAAGUAAUCCUc -3' miRNA: 3'- -CAGGGGCGUCGCGAAUUUCAUUAGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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