Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5131 | 3' | -57.9 | NC_001798.1 | + | 65229 | 0.66 | 0.825688 |
Target: 5'- --aCUGGAuCGcuUGGAGGCC-GGGUgGAc -3' miRNA: 3'- gagGACCU-GC--ACCUCCGGuUCCAgCU- -5' |
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5131 | 3' | -57.9 | NC_001798.1 | + | 4115 | 0.67 | 0.808701 |
Target: 5'- -cCCUGGGCG-GGcucGGCCGGGG-CGc -3' miRNA: 3'- gaGGACCUGCaCCu--CCGGUUCCaGCu -5' |
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5131 | 3' | -57.9 | NC_001798.1 | + | 30152 | 0.67 | 0.791096 |
Target: 5'- ----cGGGCG-GGGGGCCGGGGU-GAg -3' miRNA: 3'- gaggaCCUGCaCCUCCGGUUCCAgCU- -5' |
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5131 | 3' | -57.9 | NC_001798.1 | + | 121552 | 0.67 | 0.782082 |
Target: 5'- -gCCUGG-CGUGG-GGCCcguGGG-CGAc -3' miRNA: 3'- gaGGACCuGCACCuCCGGu--UCCaGCU- -5' |
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5131 | 3' | -57.9 | NC_001798.1 | + | 111179 | 0.68 | 0.725567 |
Target: 5'- gUCCuUGGGCG-GGAGG---AGGUCGAa -3' miRNA: 3'- gAGG-ACCUGCaCCUCCgguUCCAGCU- -5' |
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5131 | 3' | -57.9 | NC_001798.1 | + | 134397 | 1.08 | 0.002267 |
Target: 5'- cCUCCUGGACGUGGAGGCCAAGGUCGAc -3' miRNA: 3'- -GAGGACCUGCACCUCCGGUUCCAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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