Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5131 | 3' | -57.9 | NC_001798.1 | + | 104165 | 0.66 | 0.83393 |
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Target: 5'- -gCCUGGGCGUccAGGCaCAGGG-CGGc -3' miRNA: 3'- gaGGACCUGCAccUCCG-GUUCCaGCU- -5' |
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| 5131 | 3' | -57.9 | NC_001798.1 | + | 7797 | 0.66 | 0.83393 |
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Target: 5'- --gCUGG-CG-GGA-GCCAGGGUCGGa -3' miRNA: 3'- gagGACCuGCaCCUcCGGUUCCAGCU- -5' |
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| 5131 | 3' | -57.9 | NC_001798.1 | + | 30919 | 0.66 | 0.83393 |
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Target: 5'- -aCCUGGccgACGgugagGGcGGCgGGGGUCGGg -3' miRNA: 3'- gaGGACC---UGCa----CCuCCGgUUCCAGCU- -5' |
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| 5131 | 3' | -57.9 | NC_001798.1 | + | 58566 | 0.66 | 0.849874 |
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Target: 5'- cCUCCgGGGCGUaucGGGGCgGGGGagacUCGAc -3' miRNA: 3'- -GAGGaCCUGCAc--CUCCGgUUCC----AGCU- -5' |
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| 5131 | 3' | -57.9 | NC_001798.1 | + | 85808 | 0.66 | 0.849874 |
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Target: 5'- cCUCCggGGGCGaugacggggGGGGGCgGGGG-CGGg -3' miRNA: 3'- -GAGGa-CCUGCa--------CCUCCGgUUCCaGCU- -5' |
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| 5131 | 3' | -57.9 | NC_001798.1 | + | 136127 | 0.66 | 0.849874 |
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Target: 5'- gUUCUGGaagGCGUGuGGGGCgAGGG-CGGg -3' miRNA: 3'- gAGGACC---UGCAC-CUCCGgUUCCaGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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