Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5131 | 3' | -57.9 | NC_001798.1 | + | 78916 | 0.71 | 0.546311 |
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Target: 5'- -cCCUGGA---GGAGGCCGAGG-CGGa -3' miRNA: 3'- gaGGACCUgcaCCUCCGGUUCCaGCU- -5' |
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| 5131 | 3' | -57.9 | NC_001798.1 | + | 30750 | 0.74 | 0.399647 |
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Target: 5'- -cCCcGGacccGCGUGGAGGCCGAGG-CGGc -3' miRNA: 3'- gaGGaCC----UGCACCUCCGGUUCCaGCU- -5' |
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| 5131 | 3' | -57.9 | NC_001798.1 | + | 8298 | 0.74 | 0.391326 |
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Target: 5'- cCUCCUGGGgGgggGGGGGCgaGAGGUCa- -3' miRNA: 3'- -GAGGACCUgCa--CCUCCGg-UUCCAGcu -5' |
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| 5131 | 3' | -57.9 | NC_001798.1 | + | 139566 | 0.75 | 0.375026 |
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Target: 5'- uUCCgGGACGUGGcGGCCuugGAGGUCc- -3' miRNA: 3'- gAGGaCCUGCACCuCCGG---UUCCAGcu -5' |
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| 5131 | 3' | -57.9 | NC_001798.1 | + | 74860 | 0.77 | 0.280648 |
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Target: 5'- gUCCUGGAgGUGGGGGCagugccgguGGGUCGc -3' miRNA: 3'- gAGGACCUgCACCUCCGgu-------UCCAGCu -5' |
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| 5131 | 3' | -57.9 | NC_001798.1 | + | 134397 | 1.08 | 0.002267 |
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Target: 5'- cCUCCUGGACGUGGAGGCCAAGGUCGAc -3' miRNA: 3'- -GAGGACCUGCACCUCCGGUUCCAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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