Results 21 - 34 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5158 | 5' | -53.6 | NC_001798.1 | + | 26691 | 0.67 | 0.933701 |
Target: 5'- --aGGGGGAAGGA--AUCGGGcgUCUGu -3' miRNA: 3'- aaaCCCCUUUCCUggUGGUCCa-AGAC- -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 153684 | 0.67 | 0.933701 |
Target: 5'- --cGGGGAcgcGGGGGCCGCCGccGGcgCa- -3' miRNA: 3'- aaaCCCCU---UUCCUGGUGGU--CCaaGac -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 23557 | 0.66 | 0.938244 |
Target: 5'- --cGGGGGugcccgcGAGGGCC-CCGGGggCg- -3' miRNA: 3'- aaaCCCCU-------UUCCUGGuGGUCCaaGac -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 137480 | 0.66 | 0.938736 |
Target: 5'- gUUGGGGAGGGGG--GCCGGGg---- -3' miRNA: 3'- aAACCCCUUUCCUggUGGUCCaagac -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 39771 | 0.66 | 0.938736 |
Target: 5'- --gGGGGGAAGccGACCGCCuGGUc--- -3' miRNA: 3'- aaaCCCCUUUC--CUGGUGGuCCAagac -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 138883 | 0.66 | 0.94068 |
Target: 5'- aUUUGGGGAAGcuggucgguccgcuuGGGCCGCCcGGcgCa- -3' miRNA: 3'- -AAACCCCUUU---------------CCUGGUGGuCCaaGac -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 5635 | 0.66 | 0.943519 |
Target: 5'- --aGGGGgcGGGGCCGCaGGGUa--- -3' miRNA: 3'- aaaCCCCuuUCCUGGUGgUCCAagac -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 5773 | 0.66 | 0.943519 |
Target: 5'- --aGGGGgcGGGGCCGCaGGGUa--- -3' miRNA: 3'- aaaCCCCuuUCCUGGUGgUCCAagac -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 66211 | 0.66 | 0.948051 |
Target: 5'- --aGGGGuucAGGGCCuggGCCAGGaUCa- -3' miRNA: 3'- aaaCCCCuu-UCCUGG---UGGUCCaAGac -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 29494 | 0.66 | 0.948051 |
Target: 5'- --cGGGGAcGGGGCCGCCccgagAGGg---- -3' miRNA: 3'- aaaCCCCUuUCCUGGUGG-----UCCaagac -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 9118 | 0.66 | 0.952336 |
Target: 5'- --cGGGGGAGccGCCGCgAGGUggUCUGc -3' miRNA: 3'- aaaCCCCUUUccUGGUGgUCCA--AGAC- -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 146298 | 0.66 | 0.952336 |
Target: 5'- --cGGGGAGuGGGCgGCCGGGc---- -3' miRNA: 3'- aaaCCCCUUuCCUGgUGGUCCaagac -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 104588 | 0.66 | 0.956377 |
Target: 5'- --cGGGGAAcgucgcccGGGGCCGCC-GGUa--- -3' miRNA: 3'- aaaCCCCUU--------UCCUGGUGGuCCAagac -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 66862 | 0.66 | 0.960178 |
Target: 5'- --cGGGGAGucgccGGGgCGCCGGGgcUUGg -3' miRNA: 3'- aaaCCCCUUu----CCUgGUGGUCCaaGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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