Results 21 - 34 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5158 | 5' | -53.6 | NC_001798.1 | + | 72765 | 0.76 | 0.495717 |
Target: 5'- --cGGGGGAccAGGuCC-CCGGGUUCUGu -3' miRNA: 3'- aaaCCCCUU--UCCuGGuGGUCCAAGAC- -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 85976 | 0.74 | 0.577367 |
Target: 5'- --cGGGGAguucGAGGccGCCGCCGGGgcgCUGg -3' miRNA: 3'- aaaCCCCU----UUCC--UGGUGGUCCaa-GAC- -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 88038 | 0.69 | 0.845689 |
Target: 5'- --cGGGGAGggcAGGGCCGCUGGGg---- -3' miRNA: 3'- aaaCCCCUU---UCCUGGUGGUCCaagac -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 95744 | 0.73 | 0.629945 |
Target: 5'- gUUUGGGG-AGGGACCACCggagaaaaAGGacCUGa -3' miRNA: 3'- -AAACCCCuUUCCUGGUGG--------UCCaaGAC- -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 104588 | 0.66 | 0.956377 |
Target: 5'- --cGGGGAAcgucgcccGGGGCCGCC-GGUa--- -3' miRNA: 3'- aaaCCCCUU--------UCCUGGUGGuCCAagac -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 112254 | 0.67 | 0.921731 |
Target: 5'- cUUUGGGGccugucccccGGACCACCAGGc---- -3' miRNA: 3'- -AAACCCCuuu-------CCUGGUGGUCCaagac -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 115383 | 1.05 | 0.007124 |
Target: 5'- uUUUGGGGAAAGGACCACCAGGUUCUGc -3' miRNA: 3'- -AAACCCCUUUCCUGGUGGUCCAAGAC- -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 119886 | 0.75 | 0.546256 |
Target: 5'- --gGGGGGAucccggucGGGGCCGCCAGGUa--- -3' miRNA: 3'- aaaCCCCUU--------UCCUGGUGGUCCAagac -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 137480 | 0.66 | 0.938736 |
Target: 5'- gUUGGGGAGGGGG--GCCGGGg---- -3' miRNA: 3'- aAACCCCUUUCCUggUGGUCCaagac -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 138883 | 0.66 | 0.94068 |
Target: 5'- aUUUGGGGAAGcuggucgguccgcuuGGGCCGCCcGGcgCa- -3' miRNA: 3'- -AAACCCCUUU---------------CCUGGUGGuCCaaGac -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 146298 | 0.66 | 0.952336 |
Target: 5'- --cGGGGAGuGGGCgGCCGGGc---- -3' miRNA: 3'- aaaCCCCUUuCCUGgUGGUCCaagac -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 148090 | 0.68 | 0.877058 |
Target: 5'- --gGGGGccGGGGCCGCUAGGg---- -3' miRNA: 3'- aaaCCCCuuUCCUGGUGGUCCaagac -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 150619 | 0.67 | 0.911022 |
Target: 5'- -aUGGGGGGcuccuGGGCCGCgCGGGgcugUCUc -3' miRNA: 3'- aaACCCCUUu----CCUGGUG-GUCCa---AGAc -5' |
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5158 | 5' | -53.6 | NC_001798.1 | + | 153684 | 0.67 | 0.933701 |
Target: 5'- --cGGGGAcgcGGGGGCCGCCGccGGcgCa- -3' miRNA: 3'- aaaCCCCU---UUCCUGGUGGU--CCaaGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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