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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5161 | 3' | -52.6 | NC_001798.1 | + | 114329 | 1.1 | 0.005572 |
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Target: 5'- uGAACGAAGGGCGUGUGCAGUUCGAGGu -3' miRNA: 3'- -CUUGCUUCCCGCACACGUCAAGCUCC- -5' |
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| 5161 | 3' | -52.6 | NC_001798.1 | + | 136872 | 0.66 | 0.984654 |
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Target: 5'- cAGCG-AGGGCGUG-GCGuGgcucCGGGGa -3' miRNA: 3'- cUUGCuUCCCGCACaCGU-Caa--GCUCC- -5' |
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| 5161 | 3' | -52.6 | NC_001798.1 | + | 138501 | 0.67 | 0.96751 |
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Target: 5'- --cCGGAGGGCGUG-GCAcgcgacgaucUCGGGGc -3' miRNA: 3'- cuuGCUUCCCGCACaCGUca--------AGCUCC- -5' |
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| 5161 | 3' | -52.6 | NC_001798.1 | + | 144557 | 0.66 | 0.980692 |
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Target: 5'- cGugGggGGGCGUucgaaAGggCGAGGa -3' miRNA: 3'- cUugCuuCCCGCAcacg-UCaaGCUCC- -5' |
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| 5161 | 3' | -52.6 | NC_001798.1 | + | 146040 | 0.66 | 0.976012 |
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Target: 5'- --gUGggGGGCGUgGUGguGaaCGAuGGg -3' miRNA: 3'- cuuGCuuCCCGCA-CACguCaaGCU-CC- -5' |
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| 5161 | 3' | -52.6 | NC_001798.1 | + | 148275 | 0.69 | 0.912905 |
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Target: 5'- gGAGgGgcGGGCGUGgcggGCAGgugugcgggCGGGGu -3' miRNA: 3'- -CUUgCuuCCCGCACa---CGUCaa-------GCUCC- -5' |
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| 5161 | 3' | -52.6 | NC_001798.1 | + | 148382 | 0.69 | 0.912905 |
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Target: 5'- gGAGgGgcGGGCGUGgcggGCAGgugugcgggCGGGGu -3' miRNA: 3'- -CUUgCuuCCCGCACa---CGUCaa-------GCUCC- -5' |
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| 5161 | 3' | -52.6 | NC_001798.1 | + | 149398 | 0.75 | 0.648302 |
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Target: 5'- -cGCGggGGGCGUcggGUAG-UCGGGGg -3' miRNA: 3'- cuUGCuuCCCGCAca-CGUCaAGCUCC- -5' |
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| 5161 | 3' | -52.6 | NC_001798.1 | + | 150088 | 0.72 | 0.788285 |
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Target: 5'- gGGGCGAGGGGCGaGcGCGGggCGGcGGa -3' miRNA: 3'- -CUUGCUUCCCGCaCaCGUCaaGCU-CC- -5' |
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| 5161 | 3' | -52.6 | NC_001798.1 | + | 154301 | 0.66 | 0.984654 |
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Target: 5'- -uGCGGgcccucuuaaGGGGCGgcgGCAGgaCGGGGa -3' miRNA: 3'- cuUGCU----------UCCCGCacaCGUCaaGCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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