Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5175 | 5' | -54.6 | NC_001798.1 | + | 115614 | 0.71 | 0.741668 |
Target: 5'- cGGUGGG--UCGCCGaGUGcCAGAUGAc -3' miRNA: 3'- -CCACCCacAGCGGC-CGCuGUUUACUc -5' |
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5175 | 5' | -54.6 | NC_001798.1 | + | 4366 | 0.71 | 0.731896 |
Target: 5'- cGG-GGGgcgCGCCGGCGGCGguGGUGGu -3' miRNA: 3'- -CCaCCCacaGCGGCCGCUGU--UUACUc -5' |
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5175 | 5' | -54.6 | NC_001798.1 | + | 91243 | 0.72 | 0.712102 |
Target: 5'- aGGUGGGgGUuguagaaguucUGCCGGCGGCAAGg--- -3' miRNA: 3'- -CCACCCaCA-----------GCGGCCGCUGUUUacuc -5' |
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5175 | 5' | -54.6 | NC_001798.1 | + | 108141 | 0.73 | 0.620687 |
Target: 5'- uGGUGGGcGUUGCCGGgGGCc-AUGAc -3' miRNA: 3'- -CCACCCaCAGCGGCCgCUGuuUACUc -5' |
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5175 | 5' | -54.6 | NC_001798.1 | + | 130350 | 0.75 | 0.539755 |
Target: 5'- aGUGuGGUGUCGCCGGUGAUAc----- -3' miRNA: 3'- cCAC-CCACAGCGGCCGCUGUuuacuc -5' |
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5175 | 5' | -54.6 | NC_001798.1 | + | 105744 | 1.1 | 0.003611 |
Target: 5'- gGGUGGGUGUCGCCGGCGACAAAUGAGu -3' miRNA: 3'- -CCACCCACAGCGGCCGCUGUUUACUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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