Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5183 | 5' | -59.3 | NC_001798.1 | + | 67 | 0.67 | 0.670691 |
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Target: 5'- aAGGCGGGcGgcgGCGG-CGGGCGGGcggcagggcaGCCc -3' miRNA: 3'- -UUUGUCC-Ca--CGCCaGCCCGCCU----------UGGa -5' |
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| 5183 | 5' | -59.3 | NC_001798.1 | + | 154405 | 0.67 | 0.670691 |
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Target: 5'- aAGGCGGGcGgcgGCGG-CGGGCGGGcggcagggcaGCCc -3' miRNA: 3'- -UUUGUCC-Ca--CGCCaGCCCGCCU----------UGGa -5' |
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| 5183 | 5' | -59.3 | NC_001798.1 | + | 121777 | 0.67 | 0.680699 |
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Target: 5'- --cCAGGcGUccuCGGucUCGGGCGGGGCCg -3' miRNA: 3'- uuuGUCC-CAc--GCC--AGCCCGCCUUGGa -5' |
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| 5183 | 5' | -59.3 | NC_001798.1 | + | 31978 | 0.67 | 0.690669 |
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Target: 5'- ---gGGGGUGgGGgccaagaGGGCGGcGCCg -3' miRNA: 3'- uuugUCCCACgCCag-----CCCGCCuUGGa -5' |
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| 5183 | 5' | -59.3 | NC_001798.1 | + | 10308 | 0.67 | 0.690669 |
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Target: 5'- cGACGGGGggGgGGUUGGGgGGGgaacgcugACCg -3' miRNA: 3'- uUUGUCCCa-CgCCAGCCCgCCU--------UGGa -5' |
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| 5183 | 5' | -59.3 | NC_001798.1 | + | 142948 | 0.67 | 0.690669 |
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Target: 5'- -cACGGGGa-CGGcCGGGCaGAGCCg -3' miRNA: 3'- uuUGUCCCacGCCaGCCCGcCUUGGa -5' |
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| 5183 | 5' | -59.3 | NC_001798.1 | + | 56982 | 0.67 | 0.690669 |
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Target: 5'- cGugGGGGUGgaUGGUCagcgacggaGGGCGGAACa- -3' miRNA: 3'- uUugUCCCAC--GCCAG---------CCCGCCUUGga -5' |
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| 5183 | 5' | -59.3 | NC_001798.1 | + | 96030 | 0.66 | 0.777222 |
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Target: 5'- cGGCGGGGcggGCGGgCGGGCGacGGGCa- -3' miRNA: 3'- uUUGUCCCa--CGCCaGCCCGC--CUUGga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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