Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5198 | 3' | -60.4 | NC_001798.1 | + | 15394 | 0.66 | 0.756179 |
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Target: 5'- --gGCGCCCaccggACCCGggguugCGGGUccGGGAg -3' miRNA: 3'- guaCGCGGGca---UGGGCa-----GCUCG--CCCU- -5' |
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| 5198 | 3' | -60.4 | NC_001798.1 | + | 111807 | 0.66 | 0.762611 |
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Target: 5'- cCGUGCGCCgGUcGCgggCGUCcaggggcuggcgcgGGGCGGGAa -3' miRNA: 3'- -GUACGCGGgCA-UGg--GCAG--------------CUCGCCCU- -5' |
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| 5198 | 3' | -60.4 | NC_001798.1 | + | 33533 | 0.66 | 0.765351 |
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Target: 5'- gCAUGCacGCCCucugccCCCGgggaCGGGUGGGAg -3' miRNA: 3'- -GUACG--CGGGcau---GGGCa---GCUCGCCCU- -5' |
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| 5198 | 3' | -60.4 | NC_001798.1 | + | 98512 | 0.66 | 0.765351 |
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Target: 5'- --cGCGCCCccguagGCCCGccaugcgCGGGgGGGGc -3' miRNA: 3'- guaCGCGGGca----UGGGCa------GCUCgCCCU- -5' |
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| 5198 | 3' | -60.4 | NC_001798.1 | + | 152873 | 0.66 | 0.765351 |
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Target: 5'- --gGCgGCCCGUuaaaagcugcuAauuaCCG-CGAGCGGGAa -3' miRNA: 3'- guaCG-CGGGCA-----------Ug---GGCaGCUCGCCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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