Results 41 - 45 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5198 | 3' | -60.4 | NC_001798.1 | + | 148015 | 0.71 | 0.432932 |
Target: 5'- --gGCGCCCGUccccuuccucuACCgCGUgggcgCGGGCGGGGg -3' miRNA: 3'- guaCGCGGGCA-----------UGG-GCA-----GCUCGCCCU- -5' |
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5198 | 3' | -60.4 | NC_001798.1 | + | 15546 | 0.73 | 0.343791 |
Target: 5'- uCGUGUGCCCGgcCCCGggCGuugccgccgccgcGGCGGGGa -3' miRNA: 3'- -GUACGCGGGCauGGGCa-GC-------------UCGCCCU- -5' |
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5198 | 3' | -60.4 | NC_001798.1 | + | 24711 | 0.74 | 0.295407 |
Target: 5'- --cGCGCCCGggGCCCG-CGGGCGcGGc -3' miRNA: 3'- guaCGCGGGCa-UGGGCaGCUCGC-CCu -5' |
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5198 | 3' | -60.4 | NC_001798.1 | + | 52779 | 0.76 | 0.246143 |
Target: 5'- --gGCGcCCCGggcGCCCGU-GAGCGGGAc -3' miRNA: 3'- guaCGC-GGGCa--UGGGCAgCUCGCCCU- -5' |
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5198 | 3' | -60.4 | NC_001798.1 | + | 89910 | 1.07 | 0.001687 |
Target: 5'- uCAUGCGCCCGUACCCGUCGAGCGGGAc -3' miRNA: 3'- -GUACGCGGGCAUGGGCAGCUCGCCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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