Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5313 | 5' | -55 | NC_001798.1 | + | 120708 | 0.68 | 0.925377 |
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Target: 5'- gCCGUccGGCGaACGGcGCCGUCcaUCGUGa -3' miRNA: 3'- -GGUA--CCGCaUGCC-UGGCAGc-AGCACc -5' |
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| 5313 | 5' | -55 | NC_001798.1 | + | 59054 | 0.67 | 0.935448 |
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Target: 5'- aCAUGGCGUccgcauACGGGCCG-CG-CGa-- -3' miRNA: 3'- gGUACCGCA------UGCCUGGCaGCaGCacc -5' |
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| 5313 | 5' | -55 | NC_001798.1 | + | 154271 | 0.67 | 0.939683 |
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Target: 5'- cCCGgcUGGCGUGCgcagcccGGGCCGU-GUUGcGGg -3' miRNA: 3'- -GGU--ACCGCAUG-------CCUGGCAgCAGCaCC- -5' |
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| 5313 | 5' | -55 | NC_001798.1 | + | 11970 | 0.67 | 0.940142 |
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Target: 5'- uCCAgGGCGgauagGCgGGGCUGUgGgCGUGGu -3' miRNA: 3'- -GGUaCCGCa----UG-CCUGGCAgCaGCACC- -5' |
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| 5313 | 5' | -55 | NC_001798.1 | + | 36143 | 0.67 | 0.94461 |
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Target: 5'- gCCGguggGGCGcgGCGG-CgGUCGggGUGGg -3' miRNA: 3'- -GGUa---CCGCa-UGCCuGgCAGCagCACC- -5' |
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| 5313 | 5' | -55 | NC_001798.1 | + | 121791 | 0.66 | 0.96027 |
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Target: 5'- uCUcgGGCGgggcCGGGCCGgcCGUUGUcGGc -3' miRNA: 3'- -GGuaCCGCau--GCCUGGCa-GCAGCA-CC- -5' |
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| 5313 | 5' | -55 | NC_001798.1 | + | 101739 | 0.66 | 0.966821 |
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Target: 5'- ---cGGCGgccCGGGCCGUguccaCGUucaCGUGGg -3' miRNA: 3'- gguaCCGCau-GCCUGGCA-----GCA---GCACC- -5' |
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| 5313 | 5' | -55 | NC_001798.1 | + | 52211 | 0.65 | 0.969793 |
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Target: 5'- uCC-UGGCGgagcagACGGuCCaguggcucucgGUgGUCGUGGg -3' miRNA: 3'- -GGuACCGCa-----UGCCuGG-----------CAgCAGCACC- -5' |
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| 5313 | 5' | -55 | NC_001798.1 | + | 102082 | 0.65 | 0.969793 |
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Target: 5'- cCCAccGGCGgaUACGGcCCGUaGggggCGUGGg -3' miRNA: 3'- -GGUa-CCGC--AUGCCuGGCAgCa---GCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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