Results 41 - 43 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5323 | 3' | -53.4 | NC_001798.1 | + | 139498 | 0.75 | 0.596839 |
Target: 5'- -uUGGGUcCGUUGGGgGGGUACGGGCg -3' miRNA: 3'- ccACUCAaGCGAUCCgUUCGUGCCUG- -5' |
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5323 | 3' | -53.4 | NC_001798.1 | + | 22270 | 0.77 | 0.456959 |
Target: 5'- uGGUGuccguGUaccagUCGCUgGGGCGGGUACGGACu -3' miRNA: 3'- -CCACu----CA-----AGCGA-UCCGUUCGUGCCUG- -5' |
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5323 | 3' | -53.4 | NC_001798.1 | + | 126952 | 1.12 | 0.00329 |
Target: 5'- gGGUGAGUUCGCUAGGCAAGCACGGACu -3' miRNA: 3'- -CCACUCAAGCGAUCCGUUCGUGCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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