Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5472 | 3' | -56.6 | NC_001798.1 | + | 92921 | 0.67 | 0.83834 |
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Target: 5'- --cUGCUGUUcgaGgcggUCgucGGGCCCGCCGc -3' miRNA: 3'- ucuGCGACAA---Caa--AGa--CCCGGGCGGC- -5' |
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| 5472 | 3' | -56.6 | NC_001798.1 | + | 121543 | 0.66 | 0.86946 |
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Target: 5'- cGGCGCUGcgccUGg--CgugGGGCCCGUgGg -3' miRNA: 3'- uCUGCGACa---ACaaaGa--CCCGGGCGgC- -5' |
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| 5472 | 3' | -56.6 | NC_001798.1 | + | 23213 | 0.66 | 0.86946 |
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Target: 5'- cGACGCg---GgccgCUGGGUCCGCgGa -3' miRNA: 3'- uCUGCGacaaCaaa-GACCCGGGCGgC- -5' |
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| 5472 | 3' | -56.6 | NC_001798.1 | + | 25220 | 0.66 | 0.870196 |
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Target: 5'- cGGCGCUGacccgcc--GGCCCGCCGa -3' miRNA: 3'- uCUGCGACaacaaagacCCGGGCGGC- -5' |
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| 5472 | 3' | -56.6 | NC_001798.1 | + | 11180 | 0.66 | 0.876726 |
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Target: 5'- gGGGCGgUGggGc----GGGCCUGCCGa -3' miRNA: 3'- -UCUGCgACaaCaaagaCCCGGGCGGC- -5' |
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| 5472 | 3' | -56.6 | NC_001798.1 | + | 54129 | 0.66 | 0.883774 |
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Target: 5'- cGACGCUuaccggGUaUGUcgUCUGGGCCU-CCGa -3' miRNA: 3'- uCUGCGA------CA-ACAa-AGACCCGGGcGGC- -5' |
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| 5472 | 3' | -56.6 | NC_001798.1 | + | 77250 | 0.66 | 0.883774 |
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Target: 5'- cGGACGCgg-UGUcggccCUGGGCgCgGCCGc -3' miRNA: 3'- -UCUGCGacaACAaa---GACCCG-GgCGGC- -5' |
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| 5472 | 3' | -56.6 | NC_001798.1 | + | 25800 | 0.66 | 0.8906 |
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Target: 5'- cGGCGCcGUggaGUUcCUGGGgCUGCUGg -3' miRNA: 3'- uCUGCGaCAa--CAAaGACCCgGGCGGC- -5' |
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| 5472 | 3' | -56.6 | NC_001798.1 | + | 116524 | 0.66 | 0.8906 |
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Target: 5'- gGGGCGCUGcUGcaccgCggGGGUCCGCUu -3' miRNA: 3'- -UCUGCGACaACaaa--Ga-CCCGGGCGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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