miRNA: 3'- -UAAUAAUCCcGUUUCACGCUcGUGAc -5'
miRNA: 3'- uaauaaUCCCGUUucaCGCUCGUGAC- -5'
miRNA: 3'- uaauaaUCCCGUUUCaCGCUCGUGac -5'
miRNA: 3'- -UAAUAAUCCCGUUUCACGCUCGUGAC- -5'
miRNA: 3'- -UAAUAAUCCCGUUUCACGCUCGUGAC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5489 | 5' | -48.6 | NC_001798.1 | + | 91729 | 0.73 | 0.892111 |
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Target: 5'- uAUUAUUAGGaCAAAGUGCGAaCGCUu -3' miRNA: 3'- -UAAUAAUCCcGUUUCACGCUcGUGAc -5' |
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| 5489 | 5' | -48.6 | NC_001798.1 | + | 77702 | 0.74 | 0.861235 |
|
Target: 5'- -----cGGGGCAGca-GCGAGCGCUGg -3' miRNA: 3'- uaauaaUCCCGUUucaCGCUCGUGAC- -5' |
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| 5489 | 5' | -48.6 | NC_001798.1 | + | 150087 | 0.77 | 0.705464 |
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Target: 5'- -----gGGGGCGAGGgGCGAGCGCg- -3' miRNA: 3'- uaauaaUCCCGUUUCaCGCUCGUGac -5' |
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| 5489 | 5' | -48.6 | NC_001798.1 | + | 5704 | 1.07 | 0.015224 |
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Target: 5'- uAUUAUUAGGGCAAAGUGCGAGCGCUGg -3' miRNA: 3'- -UAAUAAUCCCGUUUCACGCUCGUGAC- -5' |
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| 5489 | 5' | -48.6 | NC_001798.1 | + | 5842 | 1.08 | 0.013515 |
|
Target: 5'- uAUUAUUAGGGCAAAGUGCGAGCACUGg -3' miRNA: 3'- -UAAUAAUCCCGUUUCACGCUCGUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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