Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5490 | 5' | -53 | NC_001798.1 | + | 138001 | 0.67 | 0.956197 |
Target: 5'- aGCUCGcgguCGCG-CGCCUGCagcuGggUCGc -3' miRNA: 3'- gCGAGUu---GUGCuGCGGACG----CuuAGUa -5' |
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5490 | 5' | -53 | NC_001798.1 | + | 97741 | 0.67 | 0.952163 |
Target: 5'- aGCUCGAgAaGAUG-CUGCGggUCAg -3' miRNA: 3'- gCGAGUUgUgCUGCgGACGCuuAGUa -5' |
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5490 | 5' | -53 | NC_001798.1 | + | 120376 | 0.67 | 0.952163 |
Target: 5'- gGCUguGCACGACGCagcggGCGAuGUCc- -3' miRNA: 3'- gCGAguUGUGCUGCGga---CGCU-UAGua -5' |
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5490 | 5' | -53 | NC_001798.1 | + | 114020 | 0.75 | 0.578943 |
Target: 5'- aGCUCGGCuuGAUggaGCCUGCGAAUCc- -3' miRNA: 3'- gCGAGUUGugCUG---CGGACGCUUAGua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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