miRNA: 3'- gCGAGuuGUGCUGCGGaC-GCuuAGUA- -5'
miRNA: 3'- -GCGAG-UUGUGCu------GCGGAc-GCUUAGUA- -5'
miRNA: 3'- gcgAGUUGUGC-UGCGGACGcu---UAGUa -5'
miRNA: 3'- gCGAGuUGUGCUGCGgaCGCuuAGUa -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5490 | 5' | -53 | NC_001798.1 | + | 136560 | 0.66 | 0.970004 |
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Target: 5'- aGCUUccCAUGACGCCgGcCGggUUAUa -3' miRNA: 3'- gCGAGuuGUGCUGCGGaC-GCuuAGUA- -5' |
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| 5490 | 5' | -53 | NC_001798.1 | + | 48874 | 0.66 | 0.970004 |
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Target: 5'- gCGCUCgGGCACGucucauuCGCCUcuCGGAUCGUu -3' miRNA: 3'- -GCGAG-UUGUGCu------GCGGAc-GCUUAGUA- -5' |
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| 5490 | 5' | -53 | NC_001798.1 | + | 65128 | 0.66 | 0.972902 |
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Target: 5'- --aUCAGCACGcACGCCUGCacggcGUCGc -3' miRNA: 3'- gcgAGUUGUGC-UGCGGACGcu---UAGUa -5' |
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| 5490 | 5' | -53 | NC_001798.1 | + | 34582 | 0.66 | 0.975592 |
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Target: 5'- gGCUC-ACGCGGCGCg-GCGucUCGg -3' miRNA: 3'- gCGAGuUGUGCUGCGgaCGCuuAGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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