miRNA: 3'- gCGAGU--UGugCUGCGGa----CGCuuAgUa -5'
miRNA: 3'- gCGAGUUGUGCUgCGGaCG--CuuAGua -5'
miRNA: 3'- gCGa--GUUGUGCuGCGGACGCu---UAGUa -5'
miRNA: 3'- gCGAGUUGugCUG---CGGACGCUUAGua -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5490 | 5' | -53 | NC_001798.1 | + | 109102 | 0.7 | 0.862003 |
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Target: 5'- gGCUCGgcGCugGACGCCcuccgGCGggUgGc -3' miRNA: 3'- gCGAGU--UGugCUGCGGa----CGCuuAgUa -5' |
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| 5490 | 5' | -53 | NC_001798.1 | + | 54691 | 0.7 | 0.837628 |
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Target: 5'- gGCUCAGCGCGAgGCC-GCcgGggUCu- -3' miRNA: 3'- gCGAGUUGUGCUgCGGaCG--CuuAGua -5' |
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| 5490 | 5' | -53 | NC_001798.1 | + | 106656 | 0.71 | 0.820382 |
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Target: 5'- gGCcaaCAGCACG-CGCCUGCGuucgGUCAg -3' miRNA: 3'- gCGa--GUUGUGCuGCGGACGCu---UAGUa -5' |
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| 5490 | 5' | -53 | NC_001798.1 | + | 114020 | 0.75 | 0.578943 |
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Target: 5'- aGCUCGGCuuGAUggaGCCUGCGAAUCc- -3' miRNA: 3'- gCGAGUUGugCUG---CGGACGCUUAGua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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