Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5493 | 3' | -57 | NC_001798.1 | + | 27566 | 0.67 | 0.836924 |
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Target: 5'- -gCGCCCGCGGG-GGagcGGCCGGc- -3' miRNA: 3'- ugGUGGGUGUCCaCCauaUCGGCCac -5' |
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| 5493 | 3' | -57 | NC_001798.1 | + | 152061 | 0.67 | 0.834454 |
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Target: 5'- cACgGCCCACGGG-GGUcccccgaccgcuuaAgcgGGCCGGg- -3' miRNA: 3'- -UGgUGGGUGUCCaCCA--------------Ua--UCGGCCac -5' |
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| 5493 | 3' | -57 | NC_001798.1 | + | 16221 | 0.67 | 0.828624 |
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Target: 5'- cGCCGCCUguGgGGGggcGGUGgGGCCGGg- -3' miRNA: 3'- -UGGUGGG--UgUCCa--CCAUaUCGGCCac -5' |
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| 5493 | 3' | -57 | NC_001798.1 | + | 146013 | 0.67 | 0.826099 |
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Target: 5'- cGCCACCCcccaaacagccaagGCGcGGUGGgg-GGCgUGGUGg -3' miRNA: 3'- -UGGUGGG--------------UGU-CCACCauaUCG-GCCAC- -5' |
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| 5493 | 3' | -57 | NC_001798.1 | + | 120793 | 0.67 | 0.820146 |
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Target: 5'- cACCACCCGCGGcauGUGGUcGUcGCauauauaGGUGa -3' miRNA: 3'- -UGGUGGGUGUC---CACCA-UAuCGg------CCAC- -5' |
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| 5493 | 3' | -57 | NC_001798.1 | + | 103702 | 0.67 | 0.819289 |
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Target: 5'- cCCACCCACGGGgcccgccguuguUGcGUGUccgccgacguccgGGCCGGg- -3' miRNA: 3'- uGGUGGGUGUCC------------AC-CAUA-------------UCGGCCac -5' |
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| 5493 | 3' | -57 | NC_001798.1 | + | 4194 | 0.67 | 0.811498 |
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Target: 5'- cCCGCCCGCGGcGUGGUcugcGgcGCUGGc- -3' miRNA: 3'- uGGUGGGUGUC-CACCA----UauCGGCCac -5' |
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| 5493 | 3' | -57 | NC_001798.1 | + | 110360 | 0.72 | 0.515995 |
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Target: 5'- uGCCACCCaacaGCAGcUGGcccaggGGCCGGUGg -3' miRNA: 3'- -UGGUGGG----UGUCcACCaua---UCGGCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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