Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5493 | 3' | -57 | NC_001798.1 | + | 85991 | 0.69 | 0.717539 |
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Target: 5'- cGCCGCCggggcgcuggCGCAGGggGGUG-AGCCGGa- -3' miRNA: 3'- -UGGUGG----------GUGUCCa-CCAUaUCGGCCac -5' |
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| 5493 | 3' | -57 | NC_001798.1 | + | 104480 | 0.69 | 0.687518 |
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Target: 5'- uCCuCCCACAGGaagaGGuUGUGGCCGcGUGc -3' miRNA: 3'- uGGuGGGUGUCCa---CC-AUAUCGGC-CAC- -5' |
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| 5493 | 3' | -57 | NC_001798.1 | + | 56390 | 0.7 | 0.674367 |
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Target: 5'- cACCugCCGCucGGUGGggucccgcggcgggGGCUGGUGg -3' miRNA: 3'- -UGGugGGUGu-CCACCaua-----------UCGGCCAC- -5' |
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| 5493 | 3' | -57 | NC_001798.1 | + | 91455 | 0.7 | 0.657085 |
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Target: 5'- cCCGCCCACccaacagcucgGGGUGGgAUGG-CGGUGc -3' miRNA: 3'- uGGUGGGUG-----------UCCACCaUAUCgGCCAC- -5' |
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| 5493 | 3' | -57 | NC_001798.1 | + | 37535 | 0.7 | 0.636682 |
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Target: 5'- cGCCccaaGCCCcaGGGUGGUGUGGCgGGa- -3' miRNA: 3'- -UGG----UGGGugUCCACCAUAUCGgCCac -5' |
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| 5493 | 3' | -57 | NC_001798.1 | + | 104036 | 0.7 | 0.636682 |
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Target: 5'- uCC-CCCACagGGGUGGUGUuuguGCgGGUGc -3' miRNA: 3'- uGGuGGGUG--UCCACCAUAu---CGgCCAC- -5' |
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| 5493 | 3' | -57 | NC_001798.1 | + | 112550 | 0.72 | 0.535634 |
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Target: 5'- uGCCGCCCGCGcGUagGGuUGUGGCCGGa- -3' miRNA: 3'- -UGGUGGGUGUcCA--CC-AUAUCGGCCac -5' |
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| 5493 | 3' | -57 | NC_001798.1 | + | 110360 | 0.72 | 0.515995 |
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Target: 5'- uGCCACCCaacaGCAGcUGGcccaggGGCCGGUGg -3' miRNA: 3'- -UGGUGGG----UGUCcACCaua---UCGGCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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