Results 41 - 49 of 49 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5495 | 5' | -55.7 | NC_001798.1 | + | 116510 | 0.71 | 0.661822 |
Target: 5'- cCGGCCUUCUCGcGgGGGCGCUg----- -3' miRNA: 3'- -GCCGGAAGGGC-CgCUCGUGGaaaaua -5' |
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5495 | 5' | -55.7 | NC_001798.1 | + | 122095 | 0.71 | 0.620456 |
Target: 5'- gGGCCUggagccgCCCGGCGgggGGCGCUUUg--- -3' miRNA: 3'- gCCGGAa------GGGCCGC---UCGUGGAAaaua -5' |
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5495 | 5' | -55.7 | NC_001798.1 | + | 109361 | 0.72 | 0.589496 |
Target: 5'- cCGGCCUggccgCCCGGgGAGCAgCg----- -3' miRNA: 3'- -GCCGGAa----GGGCCgCUCGUgGaaaaua -5' |
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5495 | 5' | -55.7 | NC_001798.1 | + | 134989 | 0.72 | 0.589496 |
Target: 5'- gGGCCUggcCCgCGGUGGGCGCCa----- -3' miRNA: 3'- gCCGGAa--GG-GCCGCUCGUGGaaaaua -5' |
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5495 | 5' | -55.7 | NC_001798.1 | + | 61192 | 0.73 | 0.538618 |
Target: 5'- uCGGCCUgcaCCGGCGcGCGCCg----- -3' miRNA: 3'- -GCCGGAag-GGCCGCuCGUGGaaaaua -5' |
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5495 | 5' | -55.7 | NC_001798.1 | + | 78962 | 0.73 | 0.52861 |
Target: 5'- uGGCCgcgUCCgGGgGGGCGCCUg---- -3' miRNA: 3'- gCCGGa--AGGgCCgCUCGUGGAaaaua -5' |
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5495 | 5' | -55.7 | NC_001798.1 | + | 30440 | 0.73 | 0.508807 |
Target: 5'- cCGGCC--CCCGGcCGAGCGCCa----- -3' miRNA: 3'- -GCCGGaaGGGCC-GCUCGUGGaaaaua -5' |
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5495 | 5' | -55.7 | NC_001798.1 | + | 93537 | 0.74 | 0.499024 |
Target: 5'- aGGagUUCgCCGGCGAGCACCUg---- -3' miRNA: 3'- gCCggAAG-GGCCGCUCGUGGAaaaua -5' |
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5495 | 5' | -55.7 | NC_001798.1 | + | 76563 | 0.75 | 0.415253 |
Target: 5'- gCGGCCUucgUCCCGGCGAccggccccGCGCCg----- -3' miRNA: 3'- -GCCGGA---AGGGCCGCU--------CGUGGaaaaua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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