Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5517 | 3' | -55.4 | NC_001798.1 | + | 123409 | 0.67 | 0.861241 |
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Target: 5'- --cGGGAGCCGcgGC-CCCGCcggGUCa- -3' miRNA: 3'- uuuUCCUUGGCuaCGcGGGCG---CAGag -5' |
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| 5517 | 3' | -55.4 | NC_001798.1 | + | 57542 | 0.67 | 0.868866 |
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Target: 5'- -cGGGGGGCgGAUGCGuUCCGCGg--- -3' miRNA: 3'- uuUUCCUUGgCUACGC-GGGCGCagag -5' |
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| 5517 | 3' | -55.4 | NC_001798.1 | + | 7572 | 0.67 | 0.876273 |
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Target: 5'- --cGGGAuCCauaacGCGCCCGCGUC-Cg -3' miRNA: 3'- uuuUCCUuGGcua--CGCGGGCGCAGaG- -5' |
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| 5517 | 3' | -55.4 | NC_001798.1 | + | 10325 | 0.67 | 0.876273 |
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Target: 5'- gGGGGGGAacgcugACCGGcgUGCguucgucacGCCCGCGUCg- -3' miRNA: 3'- -UUUUCCU------UGGCU--ACG---------CGGGCGCAGag -5' |
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| 5517 | 3' | -55.4 | NC_001798.1 | + | 74739 | 0.67 | 0.883455 |
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Target: 5'- uGGAGGGGACCc---CGCCCGCGaagccCUCg -3' miRNA: 3'- -UUUUCCUUGGcuacGCGGGCGCa----GAG- -5' |
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| 5517 | 3' | -55.4 | NC_001798.1 | + | 2575 | 0.66 | 0.890408 |
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Target: 5'- ---cGGGGCgGGggGCGUCCGCGcggCUCu -3' miRNA: 3'- uuuuCCUUGgCUa-CGCGGGCGCa--GAG- -5' |
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| 5517 | 3' | -55.4 | NC_001798.1 | + | 25723 | 0.66 | 0.890408 |
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Target: 5'- ----uGGACCGgcGCcCCCGaCGUCUCg -3' miRNA: 3'- uuuucCUUGGCuaCGcGGGC-GCAGAG- -5' |
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| 5517 | 3' | -55.4 | NC_001798.1 | + | 47862 | 0.66 | 0.890408 |
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Target: 5'- --cGGGGGCCccguggGCGCCgGCGggcgCUCg -3' miRNA: 3'- uuuUCCUUGGcua---CGCGGgCGCa---GAG- -5' |
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| 5517 | 3' | -55.4 | NC_001798.1 | + | 79393 | 0.66 | 0.921605 |
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Target: 5'- --cGGGcGCgGAguccGCGCCCGCGccccugCUCg -3' miRNA: 3'- uuuUCCuUGgCUa---CGCGGGCGCa-----GAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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