Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 5519 | 5' | -59.2 | NC_001798.1 | + | 32789 | 0.66 | 0.788954 |
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Target: 5'- gCGCCCGCGCCGGccggcgacgcccccGCggGCcAcgGGGAg- -3' miRNA: 3'- -GCGGGCGCGGCC--------------CG--CGaUuaCUCUac -5' |
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| 5519 | 5' | -59.2 | NC_001798.1 | + | 113629 | 0.66 | 0.782662 |
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Target: 5'- uCGCCUGCGCgGccGGCGCcgccAUGuGGGUGg -3' miRNA: 3'- -GCGGGCGCGgC--CCGCGau--UAC-UCUAC- -5' |
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| 5519 | 5' | -59.2 | NC_001798.1 | + | 7712 | 0.66 | 0.782662 |
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Target: 5'- uGCCCGCGgaccaCCGGGCcuuuuuucggacGgUGAUcGAGGUGu -3' miRNA: 3'- gCGGGCGC-----GGCCCG------------CgAUUA-CUCUAC- -5' |
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| 5519 | 5' | -59.2 | NC_001798.1 | + | 3774 | 0.66 | 0.782662 |
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Target: 5'- gCGCCCacacggGCGCCgGGGCGCcc--GAGGc- -3' miRNA: 3'- -GCGGG------CGCGG-CCCGCGauuaCUCUac -5' |
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| 5519 | 5' | -59.2 | NC_001798.1 | + | 153033 | 0.66 | 0.773564 |
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Target: 5'- gGCCCGCGCgGcGGCGCgcgguUGGc--- -3' miRNA: 3'- gCGGGCGCGgC-CCGCGauu--ACUcuac -5' |
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| 5519 | 5' | -59.2 | NC_001798.1 | + | 24668 | 0.66 | 0.773564 |
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Target: 5'- gCGgCCGUGCCGGG-GCUGGccGGAg- -3' miRNA: 3'- -GCgGGCGCGGCCCgCGAUUacUCUac -5' |
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| 5519 | 5' | -59.2 | NC_001798.1 | + | 75988 | 0.66 | 0.764345 |
|
Target: 5'- uGCCCGCcgccCCGGGCGCcgGcgGcAGGg- -3' miRNA: 3'- gCGGGCGc---GGCCCGCGa-UuaC-UCUac -5' |
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| 5519 | 5' | -59.2 | NC_001798.1 | + | 98861 | 0.66 | 0.755014 |
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Target: 5'- uGCCCGCccccgaCGGGCGCcAcgGuGGUGc -3' miRNA: 3'- gCGGGCGcg----GCCCGCGaUuaCuCUAC- -5' |
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| 5519 | 5' | -59.2 | NC_001798.1 | + | 81257 | 0.66 | 0.800461 |
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Target: 5'- cCGCCCGCGacccgCGGGaccuuGCgggggGGGAUGg -3' miRNA: 3'- -GCGGGCGCg----GCCCg----CGauua-CUCUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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