Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5540 | 3' | -64.1 | NC_001806.1 | + | 47052 | 0.66 | 0.549897 |
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Target: 5'- --cGUCGGgcgucccacacucUCUGGGCGGGCGGGgacgauCGCa -3' miRNA: 3'- gucCAGUC-------------AGGCCCGCCCGUCC------GCGa -5' |
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| 5540 | 3' | -64.1 | NC_001806.1 | + | 11462 | 0.66 | 0.55085 |
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Target: 5'- gAGcUCGGcUCCGaGGCGGGCcauGGcCGCUu -3' miRNA: 3'- gUCcAGUC-AGGC-CCGCCCGu--CC-GCGA- -5' |
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| 5540 | 3' | -64.1 | NC_001806.1 | + | 71516 | 0.66 | 0.55085 |
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Target: 5'- uCGGuGUUAugguGUgCGGGCGGGCGGGgGg- -3' miRNA: 3'- -GUC-CAGU----CAgGCCCGCCCGUCCgCga -5' |
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| 5540 | 3' | -64.1 | NC_001806.1 | + | 24027 | 0.66 | 0.55085 |
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Target: 5'- gCGGGcCGGacacggccgcCUGGGCGGGCAauuggaccGGCGCc -3' miRNA: 3'- -GUCCaGUCa---------GGCCCGCCCGU--------CCGCGa -5' |
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| 5540 | 3' | -64.1 | NC_001806.1 | + | 56852 | 0.65 | 0.559453 |
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Target: 5'- cCAGGgCGG-CCGcGGCGGGCccaccgauccaccAGaGCGCg -3' miRNA: 3'- -GUCCaGUCaGGC-CCGCCCG-------------UC-CGCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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