Results 21 - 40 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5540 | 3' | -64.1 | NC_001806.1 | + | 9191 | 0.67 | 0.467625 |
Target: 5'- cCGGG--GGUCgCGGGUGuGGCgaGGGCGCg -3' miRNA: 3'- -GUCCagUCAG-GCCCGC-CCG--UCCGCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 22057 | 0.67 | 0.467625 |
Target: 5'- -uGGccCGGcgCCGGGCccccgcccccgGGGCGGGUGCUg -3' miRNA: 3'- guCCa-GUCa-GGCCCG-----------CCCGUCCGCGA- -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 89258 | 0.67 | 0.475673 |
Target: 5'- gGGGUCcccggcuGGcUCCgcgaGGGCcGGCGGGCGCa -3' miRNA: 3'- gUCCAG-------UC-AGG----CCCGcCCGUCCGCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 148191 | 0.67 | 0.476571 |
Target: 5'- gCGGGgc-GUCCGgcGGgGGGCGGGCGgUa -3' miRNA: 3'- -GUCCaguCAGGC--CCgCCCGUCCGCgA- -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 2641 | 0.67 | 0.485601 |
Target: 5'- gCGGGgagCAGuaggccUCCaGGGCGGcggccGCGGGCGCc -3' miRNA: 3'- -GUCCa--GUC------AGG-CCCGCC-----CGUCCGCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 135584 | 0.67 | 0.485601 |
Target: 5'- cCAGGUCGG-CCGcGCGcuGCuGGCGCUc -3' miRNA: 3'- -GUCCAGUCaGGCcCGCc-CGuCCGCGA- -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 101400 | 0.67 | 0.494712 |
Target: 5'- -cGGUCGGg--GGGCGGGCggaaAGGCGa- -3' miRNA: 3'- guCCAGUCaggCCCGCCCG----UCCGCga -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 83276 | 0.66 | 0.502977 |
Target: 5'- gAGGg-GGUUCGGGCGGGCGcGGaggacccCGCa -3' miRNA: 3'- gUCCagUCAGGCCCGCCCGU-CC-------GCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 31953 | 0.66 | 0.51316 |
Target: 5'- uGGGagGGguggCgGGGCGGGacgGGGCGCc -3' miRNA: 3'- gUCCagUCa---GgCCCGCCCg--UCCGCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 34775 | 0.66 | 0.51316 |
Target: 5'- gCGGGgCGGUUCGGGgGGGgGGGgGg- -3' miRNA: 3'- -GUCCaGUCAGGCCCgCCCgUCCgCga -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 77375 | 0.66 | 0.51316 |
Target: 5'- aCAGGcCAGaCgGGcGCGGGCGcGGCGg- -3' miRNA: 3'- -GUCCaGUCaGgCC-CGCCCGU-CCGCga -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 15703 | 0.66 | 0.51316 |
Target: 5'- cCAGGcCGGU-CGGGUGuacGGCGGGCGa- -3' miRNA: 3'- -GUCCaGUCAgGCCCGC---CCGUCCGCga -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 24994 | 0.66 | 0.521554 |
Target: 5'- gGGGUgcuguaaCGGgCCGGGacgGGGCGgGGCGCUu -3' miRNA: 3'- gUCCA-------GUCaGGCCCg--CCCGU-CCGCGA- -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 45306 | 0.66 | 0.52249 |
Target: 5'- gCGGGgagCcGcCCGGcGaggaGGGCGGGCGCg -3' miRNA: 3'- -GUCCa--GuCaGGCC-Cg---CCCGUCCGCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 26700 | 0.66 | 0.52249 |
Target: 5'- cCGGcGUgGGcCCGGG-GGGCGGG-GCUg -3' miRNA: 3'- -GUC-CAgUCaGGCCCgCCCGUCCgCGA- -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 25863 | 0.66 | 0.531885 |
Target: 5'- -uGG-CAGcCCGGGCcccccGCGGGCGCg -3' miRNA: 3'- guCCaGUCaGGCCCGcc---CGUCCGCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 110968 | 0.66 | 0.531885 |
Target: 5'- --cGUCGG-CUacuacguuacguGGGCGGuGCGGGCGCUc -3' miRNA: 3'- gucCAGUCaGG------------CCCGCC-CGUCCGCGA- -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 5057 | 0.66 | 0.532828 |
Target: 5'- gAGGgccccccgcucgucgCGGUCUGGGCucgGGGUGGGCGg- -3' miRNA: 3'- gUCCa--------------GUCAGGCCCG---CCCGUCCGCga -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 794 | 0.66 | 0.54134 |
Target: 5'- uCAGGUCAGagauccaaacccUCCGGG-GGcGCccGCGCa -3' miRNA: 3'- -GUCCAGUC------------AGGCCCgCC-CGucCGCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 28178 | 0.66 | 0.54134 |
Target: 5'- gAGGUgAGgggCCGGGCgccaugucugGGGCgccauauugggGGGCGCc -3' miRNA: 3'- gUCCAgUCa--GGCCCG----------CCCG-----------UCCGCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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