Results 1 - 20 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5540 | 3' | -64.1 | NC_001806.1 | + | 146079 | 1.06 | 0.000853 |
Target: 5'- cCAGGUCAGUCCGGGCGGGCAGGCGCUc -3' miRNA: 3'- -GUCCAGUCAGGCCCGCCCGUCCGCGA- -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 25906 | 0.77 | 0.113745 |
Target: 5'- gCGGGcggCGGUCCGGGCGgcgugcgcgcgcgcGGCGGGCGUg -3' miRNA: 3'- -GUCCa--GUCAGGCCCGC--------------CCGUCCGCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 2726 | 0.75 | 0.157883 |
Target: 5'- gGGGUCGGggCCcucGGCGGGCcGGCGCg -3' miRNA: 3'- gUCCAGUCa-GGc--CCGCCCGuCCGCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 148395 | 0.74 | 0.178159 |
Target: 5'- -uGGUgGGcUCCGGGUGGGUGGGCGaCa -3' miRNA: 3'- guCCAgUC-AGGCCCGCCCGUCCGC-Ga -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 34421 | 0.72 | 0.241948 |
Target: 5'- cCGGGcCGGgCCGGGcCGGGUGGGCGg- -3' miRNA: 3'- -GUCCaGUCaGGCCC-GCCCGUCCGCga -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 99640 | 0.71 | 0.277324 |
Target: 5'- gGGGgcgguggCGGgCCuGGCGGGCAGGCaGCUg -3' miRNA: 3'- gUCCa------GUCaGGcCCGCCCGUCCG-CGA- -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 4659 | 0.7 | 0.323665 |
Target: 5'- -cGG-CGG-CUGGGCcGGCGGGCGCg -3' miRNA: 3'- guCCaGUCaGGCCCGcCCGUCCGCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 151444 | 0.7 | 0.323665 |
Target: 5'- gCGGGgggCGGcggCgGGGCGGccGCGGGCGCg -3' miRNA: 3'- -GUCCa--GUCa--GgCCCGCC--CGUCCGCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 3526 | 0.69 | 0.345223 |
Target: 5'- gGGGUC-GUCgccccccgCGGGgGaGGCGGGCGCg -3' miRNA: 3'- gUCCAGuCAG--------GCCCgC-CCGUCCGCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 127726 | 0.69 | 0.367793 |
Target: 5'- gGGGUCuuccgcauauucGGaaucCCGGGCGGGCugccGGGUGCg -3' miRNA: 3'- gUCCAG------------UCa---GGCCCGCCCG----UCCGCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 20714 | 0.69 | 0.375537 |
Target: 5'- gGGGUgGGcccgCCGGGgGGGCGGGgGg- -3' miRNA: 3'- gUCCAgUCa---GGCCCgCCCGUCCgCga -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 100722 | 0.69 | 0.375537 |
Target: 5'- uGGGUCAGcgccuccacgCCGGGCGccCAGGUGCg -3' miRNA: 3'- gUCCAGUCa---------GGCCCGCccGUCCGCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 42640 | 0.69 | 0.375537 |
Target: 5'- gCGGcGUCGcGUCCGGuGCggGGGCuGGCGUUg -3' miRNA: 3'- -GUC-CAGU-CAGGCC-CG--CCCGuCCGCGA- -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 4427 | 0.69 | 0.375537 |
Target: 5'- gGGGU-GGcCCGGGCGGGgGcGGCGUc -3' miRNA: 3'- gUCCAgUCaGGCCCGCCCgU-CCGCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 100372 | 0.69 | 0.386564 |
Target: 5'- aCAGGUagcucccgucgCCGGGCGGcGCGGGgGCc -3' miRNA: 3'- -GUCCAguca-------GGCCCGCC-CGUCCgCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 151102 | 0.68 | 0.432743 |
Target: 5'- gGGGUCgcgggGGUCgCGGG-GGGCuccGGCGCc -3' miRNA: 3'- gUCCAG-----UCAG-GCCCgCCCGu--CCGCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 33987 | 0.68 | 0.432743 |
Target: 5'- gGGGUCg--CCGGGgcagGGGCGGGgGCg -3' miRNA: 3'- gUCCAGucaGGCCCg---CCCGUCCgCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 151549 | 0.68 | 0.432743 |
Target: 5'- cCGGG-CGG-CCGGGgGcGGCGGGgGCc -3' miRNA: 3'- -GUCCaGUCaGGCCCgC-CCGUCCgCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 113750 | 0.68 | 0.441323 |
Target: 5'- -cGGccCGGagCUGGGCGGGCAGG-GCa -3' miRNA: 3'- guCCa-GUCa-GGCCCGCCCGUCCgCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 59141 | 0.67 | 0.467625 |
Target: 5'- gAGGgCAG-CCGGGCcgGGGUcucGGGUGCg -3' miRNA: 3'- gUCCaGUCaGGCCCG--CCCG---UCCGCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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