Results 21 - 40 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5540 | 3' | -64.1 | NC_001806.1 | + | 34421 | 0.72 | 0.241948 |
Target: 5'- cCGGGcCGGgCCGGGcCGGGUGGGCGg- -3' miRNA: 3'- -GUCCaGUCaGGCCC-GCCCGUCCGCga -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 34775 | 0.66 | 0.51316 |
Target: 5'- gCGGGgCGGUUCGGGgGGGgGGGgGg- -3' miRNA: 3'- -GUCCaGUCAGGCCCgCCCgUCCgCga -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 42640 | 0.69 | 0.375537 |
Target: 5'- gCGGcGUCGcGUCCGGuGCggGGGCuGGCGUUg -3' miRNA: 3'- -GUC-CAGU-CAGGCC-CG--CCCGuCCGCGA- -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 45306 | 0.66 | 0.52249 |
Target: 5'- gCGGGgagCcGcCCGGcGaggaGGGCGGGCGCg -3' miRNA: 3'- -GUCCa--GuCaGGCC-Cg---CCCGUCCGCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 47052 | 0.66 | 0.549897 |
Target: 5'- --cGUCGGgcgucccacacucUCUGGGCGGGCGGGgacgauCGCa -3' miRNA: 3'- gucCAGUC-------------AGGCCCGCCCGUCC------GCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 56852 | 0.65 | 0.559453 |
Target: 5'- cCAGGgCGG-CCGcGGCGGGCccaccgauccaccAGaGCGCg -3' miRNA: 3'- -GUCCaGUCaGGC-CCGCCCG-------------UC-CGCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 59141 | 0.67 | 0.467625 |
Target: 5'- gAGGgCAG-CCGGGCcgGGGUcucGGGUGCg -3' miRNA: 3'- gUCCaGUCaGGCCCG--CCCG---UCCGCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 71516 | 0.66 | 0.55085 |
Target: 5'- uCGGuGUUAugguGUgCGGGCGGGCGGGgGg- -3' miRNA: 3'- -GUC-CAGU----CAgGCCCGCCCGUCCgCga -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 77375 | 0.66 | 0.51316 |
Target: 5'- aCAGGcCAGaCgGGcGCGGGCGcGGCGg- -3' miRNA: 3'- -GUCCaGUCaGgCC-CGCCCGU-CCGCga -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 83276 | 0.66 | 0.502977 |
Target: 5'- gAGGg-GGUUCGGGCGGGCGcGGaggacccCGCa -3' miRNA: 3'- gUCCagUCAGGCCCGCCCGU-CC-------GCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 89258 | 0.67 | 0.475673 |
Target: 5'- gGGGUCcccggcuGGcUCCgcgaGGGCcGGCGGGCGCa -3' miRNA: 3'- gUCCAG-------UC-AGG----CCCGcCCGUCCGCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 99640 | 0.71 | 0.277324 |
Target: 5'- gGGGgcgguggCGGgCCuGGCGGGCAGGCaGCUg -3' miRNA: 3'- gUCCa------GUCaGGcCCGCCCGUCCG-CGA- -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 100372 | 0.69 | 0.386564 |
Target: 5'- aCAGGUagcucccgucgCCGGGCGGcGCGGGgGCc -3' miRNA: 3'- -GUCCAguca-------GGCCCGCC-CGUCCgCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 100722 | 0.69 | 0.375537 |
Target: 5'- uGGGUCAGcgccuccacgCCGGGCGccCAGGUGCg -3' miRNA: 3'- gUCCAGUCa---------GGCCCGCccGUCCGCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 101400 | 0.67 | 0.494712 |
Target: 5'- -cGGUCGGg--GGGCGGGCggaaAGGCGa- -3' miRNA: 3'- guCCAGUCaggCCCGCCCG----UCCGCga -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 110968 | 0.66 | 0.531885 |
Target: 5'- --cGUCGG-CUacuacguuacguGGGCGGuGCGGGCGCUc -3' miRNA: 3'- gucCAGUCaGG------------CCCGCC-CGUCCGCGA- -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 113750 | 0.68 | 0.441323 |
Target: 5'- -cGGccCGGagCUGGGCGGGCAGG-GCa -3' miRNA: 3'- guCCa-GUCa-GGCCCGCCCGUCCgCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 127726 | 0.69 | 0.367793 |
Target: 5'- gGGGUCuuccgcauauucGGaaucCCGGGCGGGCugccGGGUGCg -3' miRNA: 3'- gUCCAG------------UCa---GGCCCGCCCG----UCCGCGa -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 135584 | 0.67 | 0.485601 |
Target: 5'- cCAGGUCGG-CCGcGCGcuGCuGGCGCUc -3' miRNA: 3'- -GUCCAGUCaGGCcCGCc-CGuCCGCGA- -5' |
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5540 | 3' | -64.1 | NC_001806.1 | + | 146079 | 1.06 | 0.000853 |
Target: 5'- cCAGGUCAGUCCGGGCGGGCAGGCGCUc -3' miRNA: 3'- -GUCCAGUCAGGCCCGCCCGUCCGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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