Results 1 - 20 of 68 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5540 | 5' | -53.5 | NC_001806.1 | + | 135785 | 0.65 | 0.960783 |
Target: 5'- -cGGCGCCCGCccccccugguccugGCCCGcagACCCgcgGCUGUu -3' miRNA: 3'- aaUUGUGGGUG--------------UGGGU---UGGG---UGACAc -5' |
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5540 | 5' | -53.5 | NC_001806.1 | + | 26646 | 0.66 | 0.95817 |
Target: 5'- -cGGCGCCagAgGCCCGGCCCACc--- -3' miRNA: 3'- aaUUGUGGg-UgUGGGUUGGGUGacac -5' |
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5540 | 5' | -53.5 | NC_001806.1 | + | 101140 | 0.66 | 0.95817 |
Target: 5'- -cAGCACCCGC-CCCAcCUCGCa--- -3' miRNA: 3'- aaUUGUGGGUGuGGGUuGGGUGacac -5' |
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5540 | 5' | -53.5 | NC_001806.1 | + | 46123 | 0.66 | 0.95817 |
Target: 5'- -gGGCGCCUcCGCC--ACCCGCUGg- -3' miRNA: 3'- aaUUGUGGGuGUGGguUGGGUGACac -5' |
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5540 | 5' | -53.5 | NC_001806.1 | + | 52157 | 0.66 | 0.95817 |
Target: 5'- ---cCACCUgacGCGCCUAGCCaacCUGUGg -3' miRNA: 3'- aauuGUGGG---UGUGGGUUGGgu-GACAC- -5' |
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5540 | 5' | -53.5 | NC_001806.1 | + | 24731 | 0.66 | 0.957015 |
Target: 5'- --uGCGCCCGCGCCCugcuggagcccgacGAcgacgccccCCCGCUGg- -3' miRNA: 3'- aauUGUGGGUGUGGG--------------UU---------GGGUGACac -5' |
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5540 | 5' | -53.5 | NC_001806.1 | + | 65589 | 0.66 | 0.954234 |
Target: 5'- -aAGCACCauCACGCCauuGACUCGCUGg- -3' miRNA: 3'- aaUUGUGG--GUGUGGg--UUGGGUGACac -5' |
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5540 | 5' | -53.5 | NC_001806.1 | + | 67862 | 0.66 | 0.954234 |
Target: 5'- --cGCACCCACGCCguccgggcuGCCCGCa--- -3' miRNA: 3'- aauUGUGGGUGUGGgu-------UGGGUGacac -5' |
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5540 | 5' | -53.5 | NC_001806.1 | + | 5045 | 0.66 | 0.950055 |
Target: 5'- --cGCGCCC-CACCCGAgggcccCCCGCUcGUc -3' miRNA: 3'- aauUGUGGGuGUGGGUU------GGGUGA-CAc -5' |
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5540 | 5' | -53.5 | NC_001806.1 | + | 145447 | 0.66 | 0.945629 |
Target: 5'- gUAACccCCCACGCCaauGACCCACg--- -3' miRNA: 3'- aAUUGu-GGGUGUGGg--UUGGGUGacac -5' |
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5540 | 5' | -53.5 | NC_001806.1 | + | 23817 | 0.66 | 0.945629 |
Target: 5'- uUUGGCcCCCugcGCGCCUcgGGCCCGCUGc- -3' miRNA: 3'- -AAUUGuGGG---UGUGGG--UUGGGUGACac -5' |
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5540 | 5' | -53.5 | NC_001806.1 | + | 35727 | 0.66 | 0.945629 |
Target: 5'- ---cCACCCcccaACCUAugCCACUGUc -3' miRNA: 3'- aauuGUGGGug--UGGGUugGGUGACAc -5' |
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5540 | 5' | -53.5 | NC_001806.1 | + | 14777 | 0.66 | 0.940952 |
Target: 5'- --cGC-CCCAUACCCuACCCGCg--- -3' miRNA: 3'- aauUGuGGGUGUGGGuUGGGUGacac -5' |
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5540 | 5' | -53.5 | NC_001806.1 | + | 130146 | 0.66 | 0.940471 |
Target: 5'- -aGugGCCCGCGcaguucuCCCAggucacaaACCUGCUGUGu -3' miRNA: 3'- aaUugUGGGUGU-------GGGU--------UGGGUGACAC- -5' |
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5540 | 5' | -53.5 | NC_001806.1 | + | 62646 | 0.67 | 0.936024 |
Target: 5'- -gAGCGCCUGCGCCaCGGaCCACUGc- -3' miRNA: 3'- aaUUGUGGGUGUGG-GUUgGGUGACac -5' |
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5540 | 5' | -53.5 | NC_001806.1 | + | 36872 | 0.67 | 0.936024 |
Target: 5'- -cGugACCCACGCCCcGCCgGCc--- -3' miRNA: 3'- aaUugUGGGUGUGGGuUGGgUGacac -5' |
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5540 | 5' | -53.5 | NC_001806.1 | + | 144310 | 0.67 | 0.936024 |
Target: 5'- -cGACACCCcCcCCCcGCCCG-UGUGg -3' miRNA: 3'- aaUUGUGGGuGuGGGuUGGGUgACAC- -5' |
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5540 | 5' | -53.5 | NC_001806.1 | + | 27313 | 0.67 | 0.936024 |
Target: 5'- -cAGCGUCCGCcgagucuucgggGCCCGGCCCAUUGg- -3' miRNA: 3'- aaUUGUGGGUG------------UGGGUUGGGUGACac -5' |
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5540 | 5' | -53.5 | NC_001806.1 | + | 8491 | 0.67 | 0.936024 |
Target: 5'- ---cCACCCACucCCCAcccACCCACUc-- -3' miRNA: 3'- aauuGUGGGUGu-GGGU---UGGGUGAcac -5' |
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5540 | 5' | -53.5 | NC_001806.1 | + | 8431 | 0.67 | 0.936024 |
Target: 5'- ---cCACCCACucCCCAcccACCCACUc-- -3' miRNA: 3'- aauuGUGGGUGu-GGGU---UGGGUGAcac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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