Results 1 - 20 of 90 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5544 | 3' | -62 | NC_001806.1 | + | 42018 | 0.66 | 0.702454 |
Target: 5'- gCUGGCcGCGGggUUGGGGUCguggguCGUCaCGg -3' miRNA: 3'- -GGCCGcCGUCuuGGCCCCAG------GCAG-GU- -5' |
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5544 | 3' | -62 | NC_001806.1 | + | 78921 | 0.66 | 0.702454 |
Target: 5'- gCCGaGCGGCcccucgcCCGGGGcCCG-CCGu -3' miRNA: 3'- -GGC-CGCCGucuu---GGCCCCaGGCaGGU- -5' |
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5544 | 3' | -62 | NC_001806.1 | + | 59132 | 0.66 | 0.702454 |
Target: 5'- uUCGGUGGUGagggcagccGGGCCGGGGUCUcggGUgCGg -3' miRNA: 3'- -GGCCGCCGU---------CUUGGCCCCAGG---CAgGU- -5' |
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5544 | 3' | -62 | NC_001806.1 | + | 127516 | 0.66 | 0.702454 |
Target: 5'- aCGGCGGaCGGugcuguACaCGGGGUCC-UCg- -3' miRNA: 3'- gGCCGCC-GUCu-----UG-GCCCCAGGcAGgu -5' |
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5544 | 3' | -62 | NC_001806.1 | + | 119750 | 0.66 | 0.702454 |
Target: 5'- gCCGGCGGCGacggccGGACUGGcuguGGagCCGUCg- -3' miRNA: 3'- -GGCCGCCGU------CUUGGCC----CCa-GGCAGgu -5' |
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5544 | 3' | -62 | NC_001806.1 | + | 136994 | 0.66 | 0.702454 |
Target: 5'- aUGGCGGCGGccGgCGGGGagCG-CCAg -3' miRNA: 3'- gGCCGCCGUCu-UgGCCCCagGCaGGU- -5' |
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5544 | 3' | -62 | NC_001806.1 | + | 3511 | 0.66 | 0.692918 |
Target: 5'- gUGGCgGGCGGcgUCGGGGU-CGUCg- -3' miRNA: 3'- gGCCG-CCGUCuuGGCCCCAgGCAGgu -5' |
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5544 | 3' | -62 | NC_001806.1 | + | 24537 | 0.66 | 0.692918 |
Target: 5'- gCCgGGCGGCGGccUCGGGGaccaCCGacgCCAu -3' miRNA: 3'- -GG-CCGCCGUCuuGGCCCCa---GGCa--GGU- -5' |
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5544 | 3' | -62 | NC_001806.1 | + | 57756 | 0.66 | 0.692918 |
Target: 5'- gCGGCGGgAcGGGCCGccGUCCcGUCCGc -3' miRNA: 3'- gGCCGCCgU-CUUGGCccCAGG-CAGGU- -5' |
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5544 | 3' | -62 | NC_001806.1 | + | 96455 | 0.66 | 0.691962 |
Target: 5'- gUCGGCGGCuccGAguucccccggcacGCCuGGGGUCgCGgCCGc -3' miRNA: 3'- -GGCCGCCGu--CU-------------UGG-CCCCAG-GCaGGU- -5' |
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5544 | 3' | -62 | NC_001806.1 | + | 18376 | 0.66 | 0.683338 |
Target: 5'- -aGGCGGUGGAGaagcgcauuuuCCGGcagCCGUCCAg -3' miRNA: 3'- ggCCGCCGUCUU-----------GGCCccaGGCAGGU- -5' |
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5544 | 3' | -62 | NC_001806.1 | + | 5105 | 0.66 | 0.683338 |
Target: 5'- gUCGGUggGGCccggGGAGCCGGGGcgcugcuuguucUCCGacgCCAu -3' miRNA: 3'- -GGCCG--CCG----UCUUGGCCCC------------AGGCa--GGU- -5' |
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5544 | 3' | -62 | NC_001806.1 | + | 78672 | 0.66 | 0.683338 |
Target: 5'- gCUGGCGGCAGugcuCCuGGGcgcgcCCGUCg- -3' miRNA: 3'- -GGCCGCCGUCuu--GGcCCCa----GGCAGgu -5' |
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5544 | 3' | -62 | NC_001806.1 | + | 94541 | 0.66 | 0.683338 |
Target: 5'- -gGGCGGUGGggUCGGGccuuaUCGUCCc -3' miRNA: 3'- ggCCGCCGUCuuGGCCCca---GGCAGGu -5' |
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5544 | 3' | -62 | NC_001806.1 | + | 49452 | 0.66 | 0.673719 |
Target: 5'- cCCGGCaGGgGGGauACCuGGGUcCCGUCg- -3' miRNA: 3'- -GGCCG-CCgUCU--UGGcCCCA-GGCAGgu -5' |
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5544 | 3' | -62 | NC_001806.1 | + | 68100 | 0.66 | 0.673719 |
Target: 5'- gCCGGgcgucaUGGCAG-ACCGcGGUcucCCGUCCGa -3' miRNA: 3'- -GGCC------GCCGUCuUGGCcCCA---GGCAGGU- -5' |
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5544 | 3' | -62 | NC_001806.1 | + | 27278 | 0.66 | 0.673719 |
Target: 5'- aUCGGCGGCcgccAGcGCggCGGGGcCCGgCCAa -3' miRNA: 3'- -GGCCGCCG----UCuUG--GCCCCaGGCaGGU- -5' |
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5544 | 3' | -62 | NC_001806.1 | + | 150947 | 0.66 | 0.673719 |
Target: 5'- cCCGGCGcCGGAACCGagcccGGUCgGcCCGc -3' miRNA: 3'- -GGCCGCcGUCUUGGCc----CCAGgCaGGU- -5' |
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5544 | 3' | -62 | NC_001806.1 | + | 147890 | 0.66 | 0.672755 |
Target: 5'- -gGGgGGCGGGucuguugACaagGGGGcCCGUCCGg -3' miRNA: 3'- ggCCgCCGUCU-------UGg--CCCCaGGCAGGU- -5' |
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5544 | 3' | -62 | NC_001806.1 | + | 56656 | 0.66 | 0.667932 |
Target: 5'- gCCGGCGGCccgugggaucguuggGGggUCGGGGgggaCGggggCCc -3' miRNA: 3'- -GGCCGCCG---------------UCuuGGCCCCag--GCa---GGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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