Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5544 | 5' | -51.9 | NC_001806.1 | + | 75061 | 0.66 | 0.983734 |
Target: 5'- -gUGGU-GGUgCUGAAgaaCCUGuUCGCCg -3' miRNA: 3'- caACCAuCCAgGGUUUa--GGAC-AGCGG- -5' |
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5544 | 5' | -51.9 | NC_001806.1 | + | 26533 | 0.66 | 0.983734 |
Target: 5'- --cGGUGGGcCCCcgccuUCUUGUuCGCUg -3' miRNA: 3'- caaCCAUCCaGGGuuu--AGGACA-GCGG- -5' |
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5544 | 5' | -51.9 | NC_001806.1 | + | 15197 | 0.66 | 0.981731 |
Target: 5'- --gGGUgucacGGGUCCCAucUCCccccucaagaUGuUCGCCg -3' miRNA: 3'- caaCCA-----UCCAGGGUuuAGG----------AC-AGCGG- -5' |
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5544 | 5' | -51.9 | NC_001806.1 | + | 30958 | 0.66 | 0.981731 |
Target: 5'- --cGG-GGGUCUCuagcguGGUcgcCCUGUCGCCu -3' miRNA: 3'- caaCCaUCCAGGGu-----UUA---GGACAGCGG- -5' |
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5544 | 5' | -51.9 | NC_001806.1 | + | 102808 | 0.67 | 0.972975 |
Target: 5'- --cGGUaccAGGUCCCGaacaccacccccgacGAgCCgGUCGCCc -3' miRNA: 3'- caaCCA---UCCAGGGU---------------UUaGGaCAGCGG- -5' |
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5544 | 5' | -51.9 | NC_001806.1 | + | 104195 | 0.67 | 0.971842 |
Target: 5'- --cGGcgcGGUCCCAGGUCCacuUCGCa -3' miRNA: 3'- caaCCau-CCAGGGUUUAGGac-AGCGg -5' |
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5544 | 5' | -51.9 | NC_001806.1 | + | 106812 | 0.67 | 0.968239 |
Target: 5'- --aGG-AGGgcacgaacuacgCCCAGuUCCUGUCGCg -3' miRNA: 3'- caaCCaUCCa-----------GGGUUuAGGACAGCGg -5' |
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5544 | 5' | -51.9 | NC_001806.1 | + | 84900 | 0.68 | 0.965661 |
Target: 5'- -gUGGUgcgucGGGccUCCCGGAagagCCUGcCGCCu -3' miRNA: 3'- caACCA-----UCC--AGGGUUUa---GGACaGCGG- -5' |
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5544 | 5' | -51.9 | NC_001806.1 | + | 2687 | 0.68 | 0.950564 |
Target: 5'- -gUGGcUGGGcCCCGGGggCUGcCGCCg -3' miRNA: 3'- caACC-AUCCaGGGUUUagGACaGCGG- -5' |
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5544 | 5' | -51.9 | NC_001806.1 | + | 147299 | 0.69 | 0.931578 |
Target: 5'- --aGGgGGGUCCCGGGcccacCCUGgccgCGCCc -3' miRNA: 3'- caaCCaUCCAGGGUUUa----GGACa---GCGG- -5' |
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5544 | 5' | -51.9 | NC_001806.1 | + | 100620 | 0.69 | 0.926204 |
Target: 5'- --aGGUAGGuguguccggcgaUCCCGg--CCUGcCGCCg -3' miRNA: 3'- caaCCAUCC------------AGGGUuuaGGACaGCGG- -5' |
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5544 | 5' | -51.9 | NC_001806.1 | + | 9807 | 0.69 | 0.920578 |
Target: 5'- --gGGUGGGcUCggCCAAAUCCg--CGCCg -3' miRNA: 3'- caaCCAUCC-AG--GGUUUAGGacaGCGG- -5' |
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5544 | 5' | -51.9 | NC_001806.1 | + | 42858 | 0.72 | 0.834242 |
Target: 5'- -cUGGUGGGUCCCAcccacuUCC-GUCcCCa -3' miRNA: 3'- caACCAUCCAGGGUuu----AGGaCAGcGG- -5' |
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5544 | 5' | -51.9 | NC_001806.1 | + | 142273 | 1.12 | 0.004682 |
Target: 5'- cGUUGGUAGGUCCCAAAUCCUGUCGCCc -3' miRNA: 3'- -CAACCAUCCAGGGUUUAGGACAGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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