Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5546 | 5' | -60 | NC_001806.1 | + | 5442 | 0.66 | 0.782189 |
Target: 5'- cGCUCCGUGUg-GACGaUCGGGG-CGUc -3' miRNA: 3'- -CGGGGCGCGaaCUGCcAGUCCCaGCA- -5' |
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5546 | 5' | -60 | NC_001806.1 | + | 149793 | 0.66 | 0.764081 |
Target: 5'- aCCUCGCGCUgggGGCGGcccUCAGGccggCGg -3' miRNA: 3'- cGGGGCGCGAa--CUGCC---AGUCCca--GCa -5' |
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5546 | 5' | -60 | NC_001806.1 | + | 740 | 0.66 | 0.754859 |
Target: 5'- cGUCCCGCGCUcccuCGGg-GGGGuUCGg -3' miRNA: 3'- -CGGGGCGCGAacu-GCCagUCCC-AGCa -5' |
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5546 | 5' | -60 | NC_001806.1 | + | 88809 | 0.66 | 0.754859 |
Target: 5'- gGCCCaggaGCGUgUGACGGUCcccGUCGg -3' miRNA: 3'- -CGGGg---CGCGaACUGCCAGuccCAGCa -5' |
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5546 | 5' | -60 | NC_001806.1 | + | 24067 | 0.66 | 0.745536 |
Target: 5'- cGCCCCcgacgugucgGCGCUgGGCGcaCAGGG-CGUg -3' miRNA: 3'- -CGGGG----------CGCGAaCUGCcaGUCCCaGCA- -5' |
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5546 | 5' | -60 | NC_001806.1 | + | 41815 | 0.67 | 0.726618 |
Target: 5'- gGCCCCGCaauacGCgggGGCGGgcgUCAGGGcccCGg -3' miRNA: 3'- -CGGGGCG-----CGaa-CUGCC---AGUCCCa--GCa -5' |
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5546 | 5' | -60 | NC_001806.1 | + | 144348 | 0.67 | 0.717041 |
Target: 5'- cGCCCCGCGC----CGGUCcauuaAGGGcgcgCGUg -3' miRNA: 3'- -CGGGGCGCGaacuGCCAG-----UCCCa---GCA- -5' |
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5546 | 5' | -60 | NC_001806.1 | + | 84233 | 0.67 | 0.707396 |
Target: 5'- gGCCaCCGUGCUgaaacACGGcCGGGGgcgCGg -3' miRNA: 3'- -CGG-GGCGCGAac---UGCCaGUCCCa--GCa -5' |
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5546 | 5' | -60 | NC_001806.1 | + | 41424 | 0.67 | 0.707396 |
Target: 5'- uGCCCaGCGCgccgUGGUCGGGGUgCGc -3' miRNA: 3'- -CGGGgCGCGaacuGCCAGUCCCA-GCa -5' |
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5546 | 5' | -60 | NC_001806.1 | + | 139805 | 0.67 | 0.687935 |
Target: 5'- gGCaCCCGCGCgaucAUGGUaCGGGGcCGg -3' miRNA: 3'- -CG-GGGCGCGaac-UGCCA-GUCCCaGCa -5' |
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5546 | 5' | -60 | NC_001806.1 | + | 20594 | 0.67 | 0.687935 |
Target: 5'- cGCCCCGCccauggcagaugGCgcgGAUGGgCGGGGcCGg -3' miRNA: 3'- -CGGGGCG------------CGaa-CUGCCaGUCCCaGCa -5' |
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5546 | 5' | -60 | NC_001806.1 | + | 147229 | 0.67 | 0.678136 |
Target: 5'- gGCCCC-CGC--GGgGGUCGGGGcCGa -3' miRNA: 3'- -CGGGGcGCGaaCUgCCAGUCCCaGCa -5' |
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5546 | 5' | -60 | NC_001806.1 | + | 133414 | 0.67 | 0.678136 |
Target: 5'- gGgCCCGCGUacaccacGGCGGcaGGGGUCGUa -3' miRNA: 3'- -CgGGGCGCGaa-----CUGCCagUCCCAGCA- -5' |
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5546 | 5' | -60 | NC_001806.1 | + | 9745 | 0.68 | 0.658439 |
Target: 5'- cGCCCCuaaccgcaggGCGCc-GugGGUUGGGGgcgCGUg -3' miRNA: 3'- -CGGGG----------CGCGaaCugCCAGUCCCa--GCA- -5' |
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5546 | 5' | -60 | NC_001806.1 | + | 57405 | 0.68 | 0.648557 |
Target: 5'- aUCCCGCGCcuuggccgUGAUGGcacgCGGGGUgGUu -3' miRNA: 3'- cGGGGCGCGa-------ACUGCCa---GUCCCAgCA- -5' |
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5546 | 5' | -60 | NC_001806.1 | + | 41215 | 0.68 | 0.618867 |
Target: 5'- aGCCCCGCGCcuaaaGugGccCAGGGccUCGUg -3' miRNA: 3'- -CGGGGCGCGaa---CugCcaGUCCC--AGCA- -5' |
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5546 | 5' | -60 | NC_001806.1 | + | 20661 | 0.69 | 0.60898 |
Target: 5'- gGCCCCcgGCGUgccgGcguCGGggCGGGGUCGUg -3' miRNA: 3'- -CGGGG--CGCGaa--Cu--GCCa-GUCCCAGCA- -5' |
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5546 | 5' | -60 | NC_001806.1 | + | 3485 | 0.69 | 0.606017 |
Target: 5'- cGCCCgGCGUcgUcgucggcgucggcgUGGCGGgcggcgUCGGGGUCGUc -3' miRNA: 3'- -CGGGgCGCG--A--------------ACUGCC------AGUCCCAGCA- -5' |
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5546 | 5' | -60 | NC_001806.1 | + | 5060 | 0.69 | 0.589261 |
Target: 5'- gGCCCCcCGCUcGucGCGGUCuGGGcUCGg -3' miRNA: 3'- -CGGGGcGCGAaC--UGCCAGuCCC-AGCa -5' |
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5546 | 5' | -60 | NC_001806.1 | + | 20979 | 0.69 | 0.559916 |
Target: 5'- aGUUCCGCGUc-GACGGcgGGGGUCGUc -3' miRNA: 3'- -CGGGGCGCGaaCUGCCagUCCCAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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