Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5550 | 5' | -54.5 | NC_001806.1 | + | 146181 | 0.68 | 0.857936 |
Target: 5'- gGUGGUCaggcagcccGGGCCGCGGcucugugguuaACACCAg--- -3' miRNA: 3'- -CACCAGa--------CCUGGCGCC-----------UGUGGUaaau -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 140068 | 1.07 | 0.005035 |
Target: 5'- cGUGGUCUGGACCGCGGACACCAUUUAc -3' miRNA: 3'- -CACCAGACCUGGCGCCUGUGGUAAAU- -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 137328 | 0.67 | 0.901094 |
Target: 5'- -aGGUCuUGGACUGUguggugacGGGCGCCAc--- -3' miRNA: 3'- caCCAG-ACCUGGCG--------CCUGUGGUaaau -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 136691 | 0.67 | 0.901094 |
Target: 5'- uUGGggCUGcaGACCGCGGGcCGCCAc--- -3' miRNA: 3'- cACCa-GAC--CUGGCGCCU-GUGGUaaau -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 132650 | 0.66 | 0.945141 |
Target: 5'- cUGGUgCgcgGGGCCGCGGccgcuuACGCCGc--- -3' miRNA: 3'- cACCA-Ga--CCUGGCGCC------UGUGGUaaau -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 131453 | 0.68 | 0.857936 |
Target: 5'- cGUGGUCguccgcGGACCgGCGG-CGCUGUUg- -3' miRNA: 3'- -CACCAGa-----CCUGG-CGCCuGUGGUAAau -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 125848 | 0.67 | 0.901094 |
Target: 5'- -cGGgcccCcGGccGCCGCGGACGCCGUg-- -3' miRNA: 3'- caCCa---GaCC--UGGCGCCUGUGGUAaau -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 125797 | 0.66 | 0.945141 |
Target: 5'- -gGGUauggUGGACCGCGGACuCgGUc-- -3' miRNA: 3'- caCCAg---ACCUGGCGCCUGuGgUAaau -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 119721 | 0.66 | 0.925099 |
Target: 5'- aGUGG-UUGGAUCGCGGAUgggaagcccugGCCGg--- -3' miRNA: 3'- -CACCaGACCUGGCGCCUG-----------UGGUaaau -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 119596 | 0.67 | 0.913589 |
Target: 5'- -gGGUCUGGAgccccCCgGCGGGgGCCGcUUUGu -3' miRNA: 3'- caCCAGACCU-----GG-CGCCUgUGGU-AAAU- -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 109248 | 0.68 | 0.849966 |
Target: 5'- -cGGaaucCUGGGCCcCGGGCACCAg--- -3' miRNA: 3'- caCCa---GACCUGGcGCCUGUGGUaaau -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 88862 | 0.68 | 0.873232 |
Target: 5'- cGUGGaaCUGGGCCGCGcGcAUGCCGUa-- -3' miRNA: 3'- -CACCa-GACCUGGCGC-C-UGUGGUAaau -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 75355 | 0.67 | 0.913589 |
Target: 5'- uUGGccCUGGACgCGCGgGugGCCAUc-- -3' miRNA: 3'- cACCa-GACCUG-GCGC-CugUGGUAaau -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 54774 | 0.66 | 0.935615 |
Target: 5'- aUGGUU--GGCCGCGGcaGCACCAg--- -3' miRNA: 3'- cACCAGacCUGGCGCC--UGUGGUaaau -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 52205 | 0.72 | 0.669291 |
Target: 5'- -cGGUacguacgGGACCGUGGAUACCGUc-- -3' miRNA: 3'- caCCAga-----CCUGGCGCCUGUGGUAaau -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 37527 | 0.66 | 0.925099 |
Target: 5'- -aGG-CUcGGGCCGUGGGCgACCAa--- -3' miRNA: 3'- caCCaGA-CCUGGCGCCUG-UGGUaaau -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 36246 | 0.66 | 0.925099 |
Target: 5'- -aGGUgCUguaGGGCCGCGGAgGCCc---- -3' miRNA: 3'- caCCA-GA---CCUGGCGCCUgUGGuaaau -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 34392 | 0.69 | 0.807189 |
Target: 5'- gGUGGg-UGGGCgGCGGugGCCGg--- -3' miRNA: 3'- -CACCagACCUGgCGCCugUGGUaaau -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 30858 | 0.72 | 0.648478 |
Target: 5'- ---uUCUGGGCCGCGGGgGCCGa--- -3' miRNA: 3'- caccAGACCUGGCGCCUgUGGUaaau -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 28375 | 0.66 | 0.940501 |
Target: 5'- ------gGGACCGgGGGCGCCAUg-- -3' miRNA: 3'- caccagaCCUGGCgCCUGUGGUAaau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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