Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5550 | 5' | -54.5 | NC_001806.1 | + | 25426 | 0.7 | 0.788864 |
Target: 5'- gGUGGU--GGugCGCGGGCGCCc---- -3' miRNA: 3'- -CACCAgaCCugGCGCCUGUGGuaaau -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 6683 | 0.68 | 0.865693 |
Target: 5'- cGUGGgccCUGGAaaUgGCGGACACCu---- -3' miRNA: 3'- -CACCa--GACCU--GgCGCCUGUGGuaaau -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 5076 | 0.66 | 0.945141 |
Target: 5'- -cGGUCUGGGCUcgggGUGGGCGgCGg--- -3' miRNA: 3'- caCCAGACCUGG----CGCCUGUgGUaaau -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 4662 | 0.68 | 0.880546 |
Target: 5'- -cGG-CUGGGCCgGCGGGCGCgGc--- -3' miRNA: 3'- caCCaGACCUGG-CGCCUGUGgUaaau -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 2661 | 0.68 | 0.880546 |
Target: 5'- -aGGgcgGcGGCCGCGGGCGCCGc--- -3' miRNA: 3'- caCCagaC-CUGGCGCCUGUGGUaaau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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