Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5550 | 5' | -54.5 | NC_001806.1 | + | 132650 | 0.66 | 0.945141 |
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Target: 5'- cUGGUgCgcgGGGCCGCGGccgcuuACGCCGc--- -3' miRNA: 3'- cACCA-Ga--CCUGGCGCC------UGUGGUaaau -5' |
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| 5550 | 5' | -54.5 | NC_001806.1 | + | 136691 | 0.67 | 0.901094 |
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Target: 5'- uUGGggCUGcaGACCGCGGGcCGCCAc--- -3' miRNA: 3'- cACCa-GAC--CUGGCGCCU-GUGGUaaau -5' |
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| 5550 | 5' | -54.5 | NC_001806.1 | + | 137328 | 0.67 | 0.901094 |
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Target: 5'- -aGGUCuUGGACUGUguggugacGGGCGCCAc--- -3' miRNA: 3'- caCCAG-ACCUGGCG--------CCUGUGGUaaau -5' |
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| 5550 | 5' | -54.5 | NC_001806.1 | + | 140068 | 1.07 | 0.005035 |
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Target: 5'- cGUGGUCUGGACCGCGGACACCAUUUAc -3' miRNA: 3'- -CACCAGACCUGGCGCCUGUGGUAAAU- -5' |
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| 5550 | 5' | -54.5 | NC_001806.1 | + | 146181 | 0.68 | 0.857936 |
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Target: 5'- gGUGGUCaggcagcccGGGCCGCGGcucugugguuaACACCAg--- -3' miRNA: 3'- -CACCAGa--------CCUGGCGCC-----------UGUGGUaaau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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