Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5551 | 5' | -56.8 | NC_001806.1 | + | 91205 | 0.66 | 0.882079 |
Target: 5'- cGUACcuGGCGCgcgCCGCGgGAcUCGUGGg- -3' miRNA: 3'- uCAUG--CCGUG---GGCGCgCU-AGUACCau -5' |
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5551 | 5' | -56.8 | NC_001806.1 | + | 115041 | 0.68 | 0.763827 |
Target: 5'- --cGCGGCAgCCGUguaccugcggaacGCGGUCGUGGc- -3' miRNA: 3'- ucaUGCCGUgGGCG-------------CGCUAGUACCau -5' |
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5551 | 5' | -56.8 | NC_001806.1 | + | 136965 | 0.68 | 0.774201 |
Target: 5'- uAGUG-GGgGCCCGCgagcgugguGCGGUCAUGGc- -3' miRNA: 3'- -UCAUgCCgUGGGCG---------CGCUAGUACCau -5' |
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5551 | 5' | -56.8 | NC_001806.1 | + | 30216 | 0.67 | 0.801676 |
Target: 5'- --cGCGcGCcCCCGCGCGGcCGUGGc- -3' miRNA: 3'- ucaUGC-CGuGGGCGCGCUaGUACCau -5' |
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5551 | 5' | -56.8 | NC_001806.1 | + | 56654 | 0.67 | 0.810539 |
Target: 5'- cGgcCGGCgGCCCGUGgGAUCGuUGGg- -3' miRNA: 3'- uCauGCCG-UGGGCGCgCUAGU-ACCau -5' |
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5551 | 5' | -56.8 | NC_001806.1 | + | 74067 | 0.67 | 0.810539 |
Target: 5'- aGGUGCGGacccagGCCCGUcggGCGAUCGaGGg- -3' miRNA: 3'- -UCAUGCCg-----UGGGCG---CGCUAGUaCCau -5' |
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5551 | 5' | -56.8 | NC_001806.1 | + | 96248 | 0.67 | 0.819239 |
Target: 5'- uGUAuCGGCcCCCG-GuCGGUCGUGGUc -3' miRNA: 3'- uCAU-GCCGuGGGCgC-GCUAGUACCAu -5' |
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5551 | 5' | -56.8 | NC_001806.1 | + | 51024 | 0.67 | 0.826923 |
Target: 5'- --aGCGGCggguccguguuggGCCCGCGgGuccgCGUGGUGg -3' miRNA: 3'- ucaUGCCG-------------UGGGCGCgCua--GUACCAU- -5' |
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5551 | 5' | -56.8 | NC_001806.1 | + | 23020 | 0.67 | 0.827768 |
Target: 5'- uGGUGCucauGCGCCUGCGCGGggaccugcgCGUGGc- -3' miRNA: 3'- -UCAUGc---CGUGGGCGCGCUa--------GUACCau -5' |
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5551 | 5' | -56.8 | NC_001806.1 | + | 5402 | 0.68 | 0.74558 |
Target: 5'- --gGCGGU-CCCGCGUcgGGUCGUGGa- -3' miRNA: 3'- ucaUGCCGuGGGCGCG--CUAGUACCau -5' |
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5551 | 5' | -56.8 | NC_001806.1 | + | 105088 | 0.68 | 0.74558 |
Target: 5'- cGGUGCGGUAUCUGCaggGCGGcgggUCGUGGc- -3' miRNA: 3'- -UCAUGCCGUGGGCG---CGCU----AGUACCau -5' |
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5551 | 5' | -56.8 | NC_001806.1 | + | 102274 | 0.69 | 0.725986 |
Target: 5'- --cGCGGCGgCgGCGCGGuuuaugucgUCGUGGUGc -3' miRNA: 3'- ucaUGCCGUgGgCGCGCU---------AGUACCAU- -5' |
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5551 | 5' | -56.8 | NC_001806.1 | + | 1754 | 0.75 | 0.403187 |
Target: 5'- --gGCGGUACUCGCGCGGggaCAUGGg- -3' miRNA: 3'- ucaUGCCGUGGGCGCGCUa--GUACCau -5' |
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5551 | 5' | -56.8 | NC_001806.1 | + | 32225 | 0.73 | 0.475361 |
Target: 5'- aGGcACGcGCACCCGCGCGGcCA-GGUGg -3' miRNA: 3'- -UCaUGC-CGUGGGCGCGCUaGUaCCAU- -5' |
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5551 | 5' | -56.8 | NC_001806.1 | + | 60501 | 0.71 | 0.573577 |
Target: 5'- --gGCGGCACCgagaCGCGCGucUCGUGGa- -3' miRNA: 3'- ucaUGCCGUGG----GCGCGCu-AGUACCau -5' |
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5551 | 5' | -56.8 | NC_001806.1 | + | 4010 | 0.71 | 0.593907 |
Target: 5'- uGUACaGCAgCCGCGUGAUCA-GGg- -3' miRNA: 3'- uCAUGcCGUgGGCGCGCUAGUaCCau -5' |
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5551 | 5' | -56.8 | NC_001806.1 | + | 92939 | 0.7 | 0.624593 |
Target: 5'- gAGgcCGGgGCCCGCGCGcUgAUGGa- -3' miRNA: 3'- -UCauGCCgUGGGCGCGCuAgUACCau -5' |
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5551 | 5' | -56.8 | NC_001806.1 | + | 16743 | 0.7 | 0.634843 |
Target: 5'- --cGCGGCAUCCGCGCuggGGUCGUccucuauggGGUAg -3' miRNA: 3'- ucaUGCCGUGGGCGCG---CUAGUA---------CCAU- -5' |
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5551 | 5' | -56.8 | NC_001806.1 | + | 140687 | 0.7 | 0.634843 |
Target: 5'- --cGCGGCGCCCGCGUGccgCGcUGGa- -3' miRNA: 3'- ucaUGCCGUGGGCGCGCua-GU-ACCau -5' |
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5551 | 5' | -56.8 | NC_001806.1 | + | 65470 | 0.69 | 0.703056 |
Target: 5'- gGGUccugGgGGCGCCCGCGCaccacgucuccgucGAugaUCAUGGUGc -3' miRNA: 3'- -UCA----UgCCGUGGGCGCG--------------CU---AGUACCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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