miRNA: 3'- -AAGACCCCGGGCUCAAACUCCAGGCC- -5'
miRNA: 3'- aaGACCCCGG-GCUCaaaCUCCaGGCC- -5'
miRNA: 3'- aagaCCCCGGGCUCaaaCUCCagGCC- -5'
miRNA: 3'- aagaCCCCGGGCuCaaa-CUCCaGGCC- -5'
miRNA: 3'- aAGa--CCCCGG-GCUCaaa----CUCCAG-GCC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5561 | 5' | -58.1 | NC_001806.1 | + | 132536 | 1.08 | 0.001974 |
|
Target: 5'- uUUCUGGGGCCCGAGUUUGAGGUCCGGg -3' miRNA: 3'- -AAGACCCCGGGCUCAAACUCCAGGCC- -5' |
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| 5561 | 5' | -58.1 | NC_001806.1 | + | 138839 | 0.76 | 0.298366 |
|
Target: 5'- -aCUGGGGCCgCGGGacgGGGGcCCGGa -3' miRNA: 3'- aaGACCCCGG-GCUCaaaCUCCaGGCC- -5' |
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| 5561 | 5' | -58.1 | NC_001806.1 | + | 147784 | 0.7 | 0.624158 |
|
Target: 5'- ---gGGGGCCCGGGgcgGGGGg-CGGa -3' miRNA: 3'- aagaCCCCGGGCUCaaaCUCCagGCC- -5' |
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| 5561 | 5' | -58.1 | NC_001806.1 | + | 147826 | 0.66 | 0.807706 |
|
Target: 5'- ---gGGGGCCUGuGgggaGAGG-CCGGg -3' miRNA: 3'- aagaCCCCGGGCuCaaa-CUCCaGGCC- -5' |
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| 5561 | 5' | -58.1 | NC_001806.1 | + | 151078 | 0.67 | 0.771736 |
|
Target: 5'- gUCgcgGGGGUCgCGGGggucgcgGGGGUCgCGGg -3' miRNA: 3'- aAGa--CCCCGG-GCUCaaa----CUCCAG-GCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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