miRNA: 3'- aAG-ACCCCgGGCUCaaaCUCCa-GGCC- -5'
miRNA: 3'- aagaCCCCGGGCuCaaa-CUCCaGGCC- -5'
miRNA: 3'- aAGaCCCCGGGcUCAAACUCCaGGCC- -5'
miRNA: 3'- aagaCCCCG----------GGCUCAAACUCCAGgCC- -5'
miRNA: 3'- aaGACCCCGGgCUCaa---ACUCCaGGCC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5561 | 5' | -58.1 | NC_001806.1 | + | 50509 | 0.66 | 0.798925 |
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Target: 5'- gUCgUGGGGgCCGAGcacGAGGcgCUGGg -3' miRNA: 3'- aAG-ACCCCgGGCUCaaaCUCCa-GGCC- -5' |
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| 5561 | 5' | -58.1 | NC_001806.1 | + | 147826 | 0.66 | 0.807706 |
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Target: 5'- ---gGGGGCCUGuGgggaGAGG-CCGGg -3' miRNA: 3'- aagaCCCCGGGCuCaaa-CUCCaGGCC- -5' |
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| 5561 | 5' | -58.1 | NC_001806.1 | + | 66553 | 0.66 | 0.816331 |
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Target: 5'- gUC-GGGGUUUcGGUUUGGGG-CCGGg -3' miRNA: 3'- aAGaCCCCGGGcUCAAACUCCaGGCC- -5' |
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| 5561 | 5' | -58.1 | NC_001806.1 | + | 55561 | 0.66 | 0.824794 |
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Target: 5'- ---gGGGGUgguguuguucUUGGGUUUGGGGUCgGGg -3' miRNA: 3'- aagaCCCCG----------GGCUCAAACUCCAGgCC- -5' |
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| 5561 | 5' | -58.1 | NC_001806.1 | + | 25634 | 0.66 | 0.8412 |
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Target: 5'- -gCUGGGGCUgGGGagggcUGGGG-CUGGg -3' miRNA: 3'- aaGACCCCGGgCUCaa---ACUCCaGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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