miRNA: 3'- aaGACCCCGGgCUCaa---ACUCCaGGCC- -5'
miRNA: 3'- aaGACCCCGGgCUCaa---ACUCCaGGCC- -5'
miRNA: 3'- aagaCCCCGGGCUcaaa----CUCCAGGCC- -5'
miRNA: 3'- aaGACCCCGG-GCUCaaaCUCCaGGCC- -5'
miRNA: 3'- -AAGACCCCGGGCUCAAACUCCAGGCC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5561 | 5' | -58.1 | NC_001806.1 | + | 25570 | 0.73 | 0.449539 |
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Target: 5'- -gCUGGGGCCgGGGagggcUGGGG-CCGGg -3' miRNA: 3'- aaGACCCCGGgCUCaa---ACUCCaGGCC- -5' |
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| 5561 | 5' | -58.1 | NC_001806.1 | + | 25602 | 0.73 | 0.449539 |
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Target: 5'- -gCUGGGGCCgGGGagggcUGGGG-CCGGg -3' miRNA: 3'- aaGACCCCGGgCUCaa---ACUCCaGGCC- -5' |
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| 5561 | 5' | -58.1 | NC_001806.1 | + | 124088 | 0.73 | 0.422933 |
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Target: 5'- ---cGGGGCCCGAagcuucggGGGGUCUGGg -3' miRNA: 3'- aagaCCCCGGGCUcaaa----CUCCAGGCC- -5' |
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| 5561 | 5' | -58.1 | NC_001806.1 | + | 138839 | 0.76 | 0.298366 |
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Target: 5'- -aCUGGGGCCgCGGGacgGGGGcCCGGa -3' miRNA: 3'- aaGACCCCGG-GCUCaaaCUCCaGGCC- -5' |
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| 5561 | 5' | -58.1 | NC_001806.1 | + | 132536 | 1.08 | 0.001974 |
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Target: 5'- uUUCUGGGGCCCGAGUUUGAGGUCCGGg -3' miRNA: 3'- -AAGACCCCGGGCUCAAACUCCAGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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