Results 21 - 28 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5563 | 3' | -55 | NC_001806.1 | + | 41620 | 0.67 | 0.920082 |
Target: 5'- cGGGGgUGUGGGGcc-CUGCuGUUCCGu -3' miRNA: 3'- -CCUCgACGCCCUaaaGAUGcCGAGGC- -5' |
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5563 | 3' | -55 | NC_001806.1 | + | 139098 | 0.66 | 0.92559 |
Target: 5'- cGGGGCUGCGaGAUg-CUGacCGGCUCg- -3' miRNA: 3'- -CCUCGACGCcCUAaaGAU--GCCGAGgc -5' |
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5563 | 3' | -55 | NC_001806.1 | + | 151447 | 0.66 | 0.930857 |
Target: 5'- gGGGGCggcgGCGGGGcggccgCggGCGcGCUCCu -3' miRNA: 3'- -CCUCGa---CGCCCUaaa---Ga-UGC-CGAGGc -5' |
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5563 | 3' | -55 | NC_001806.1 | + | 25636 | 0.66 | 0.940673 |
Target: 5'- uGGGGCUG-GGGAgggCUGgGGCUg-- -3' miRNA: 3'- -CCUCGACgCCCUaaaGAUgCCGAggc -5' |
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5563 | 3' | -55 | NC_001806.1 | + | 133342 | 0.66 | 0.940673 |
Target: 5'- uGGAGCcagGCGGGAg---UGUGGUUCUGg -3' miRNA: 3'- -CCUCGa--CGCCCUaaagAUGCCGAGGC- -5' |
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5563 | 3' | -55 | NC_001806.1 | + | 21764 | 0.66 | 0.940673 |
Target: 5'- cGGGCcgucggGCGGGGUccgUCgagcGCGGCgCCGg -3' miRNA: 3'- cCUCGa-----CGCCCUAa--AGa---UGCCGaGGC- -5' |
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5563 | 3' | -55 | NC_001806.1 | + | 39187 | 0.66 | 0.940673 |
Target: 5'- cGGGGCgGCGGccuugucuGcgUUCUugGGggCCGg -3' miRNA: 3'- -CCUCGaCGCC--------CuaAAGAugCCgaGGC- -5' |
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5563 | 3' | -55 | NC_001806.1 | + | 87396 | 0.65 | 0.94954 |
Target: 5'- cGGGCUGCGGccggacgCUugGGCgCCu -3' miRNA: 3'- cCUCGACGCCcuaaa--GAugCCGaGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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