Results 21 - 28 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5563 | 3' | -55 | NC_001806.1 | + | 20727 | 0.7 | 0.783499 |
Target: 5'- cGGGGggGCGGGGggccgGCGGcCUCCGc -3' miRNA: 3'- -CCUCgaCGCCCUaaagaUGCC-GAGGC- -5' |
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5563 | 3' | -55 | NC_001806.1 | + | 96433 | 0.68 | 0.856943 |
Target: 5'- uGAcGCUGgGGGuccugguggcgUCgGCGGCUCCGa -3' miRNA: 3'- cCU-CGACgCCCuaa--------AGaUGCCGAGGC- -5' |
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5563 | 3' | -55 | NC_001806.1 | + | 89779 | 0.68 | 0.867592 |
Target: 5'- cGGGGC-GUGGGAggggCUGgGGCggaCCGg -3' miRNA: 3'- -CCUCGaCGCCCUaaa-GAUgCCGa--GGC- -5' |
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5563 | 3' | -55 | NC_001806.1 | + | 151559 | 0.68 | 0.888991 |
Target: 5'- gGGGGCgGCGGGGgccgCgaugGCGGCggCGg -3' miRNA: 3'- -CCUCGaCGCCCUaaa-Ga---UGCCGagGC- -5' |
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5563 | 3' | -55 | NC_001806.1 | + | 24136 | 0.68 | 0.888991 |
Target: 5'- cGGGGCcGUGGaGUUUCUGgGGCUgCu -3' miRNA: 3'- -CCUCGaCGCCcUAAAGAUgCCGAgGc -5' |
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5563 | 3' | -55 | NC_001806.1 | + | 98212 | 0.67 | 0.894358 |
Target: 5'- gGGcAGCUGgGGGAgaacaacgagCUGCGGCUgaCGc -3' miRNA: 3'- -CC-UCGACgCCCUaaa-------GAUGCCGAg-GC- -5' |
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5563 | 3' | -55 | NC_001806.1 | + | 2764 | 0.67 | 0.902131 |
Target: 5'- cGGGGCgcggGCGGGccugCgccgcgGCGGC-CCGg -3' miRNA: 3'- -CCUCGa---CGCCCuaaaGa-----UGCCGaGGC- -5' |
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5563 | 3' | -55 | NC_001806.1 | + | 87396 | 0.65 | 0.94954 |
Target: 5'- cGGGCUGCGGccggacgCUugGGCgCCu -3' miRNA: 3'- cCUCGACGCCcuaaa--GAugCCGaGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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