Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5577 | 5' | -56.6 | NC_001806.1 | + | 49423 | 0.71 | 0.569853 |
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Target: 5'- uGAUGaGGUuccCCGCGGUccuccgcAGGCCCGGCAg -3' miRNA: 3'- uUUACcUCA---GGUGCCA-------UCCGGGUCGU- -5' |
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| 5577 | 5' | -56.6 | NC_001806.1 | + | 30643 | 0.71 | 0.581137 |
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Target: 5'- ---gGGGGgcgcggCCAgGGUGGGCCCGGg- -3' miRNA: 3'- uuuaCCUCa-----GGUgCCAUCCGGGUCgu -5' |
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| 5577 | 5' | -56.6 | NC_001806.1 | + | 151295 | 0.7 | 0.632859 |
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Target: 5'- ---gGGGGgcggCgGCGGUGGGCCgGGCc -3' miRNA: 3'- uuuaCCUCa---GgUGCCAUCCGGgUCGu -5' |
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| 5577 | 5' | -56.6 | NC_001806.1 | + | 51651 | 0.69 | 0.684555 |
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Target: 5'- --cUGGaAGUaCCugcagACGGUGGuGCCCAGCGg -3' miRNA: 3'- uuuACC-UCA-GG-----UGCCAUC-CGGGUCGU- -5' |
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| 5577 | 5' | -56.6 | NC_001806.1 | + | 43299 | 0.69 | 0.694796 |
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Target: 5'- ---cGGAG-CCGCGGcugcaggAGGCCCuGGCGg -3' miRNA: 3'- uuuaCCUCaGGUGCCa------UCCGGG-UCGU- -5' |
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| 5577 | 5' | -56.6 | NC_001806.1 | + | 34278 | 0.69 | 0.704984 |
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Target: 5'- -uGUGGGGUCCcggggGCGGgaugGGGUUUAGCGg -3' miRNA: 3'- uuUACCUCAGG-----UGCCa---UCCGGGUCGU- -5' |
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| 5577 | 5' | -56.6 | NC_001806.1 | + | 149424 | 0.68 | 0.72516 |
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Target: 5'- ---gGGGGUcCCACGGcgaccccGGGCCCuGCGu -3' miRNA: 3'- uuuaCCUCA-GGUGCCa------UCCGGGuCGU- -5' |
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| 5577 | 5' | -56.6 | NC_001806.1 | + | 127536 | 0.68 | 0.773991 |
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Target: 5'- ---cGGGGUCCuCGGcGGGCUCGGgGu -3' miRNA: 3'- uuuaCCUCAGGuGCCaUCCGGGUCgU- -5' |
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| 5577 | 5' | -56.6 | NC_001806.1 | + | 132941 | 0.66 | 0.868426 |
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Target: 5'- ---cGGAcccCCACGGccggacGGGCCUAGCAa -3' miRNA: 3'- uuuaCCUca-GGUGCCa-----UCCGGGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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