Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5577 | 5' | -56.6 | NC_001806.1 | + | 98467 | 0.66 | 0.844891 |
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Target: 5'- --cUGGAcuaCACGG-AGGUCCAGCGc -3' miRNA: 3'- uuuACCUcagGUGCCaUCCGGGUCGU- -5' |
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| 5577 | 5' | -56.6 | NC_001806.1 | + | 102202 | 0.66 | 0.868426 |
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Target: 5'- -uAUGGuGUUCGCGGUu-GCCCgcAGCAg -3' miRNA: 3'- uuUACCuCAGGUGCCAucCGGG--UCGU- -5' |
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| 5577 | 5' | -56.6 | NC_001806.1 | + | 125247 | 1.06 | 0.003172 |
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Target: 5'- gAAAUGGAGUCCACGGUAGGCCCAGCAu -3' miRNA: 3'- -UUUACCUCAGGUGCCAUCCGGGUCGU- -5' |
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| 5577 | 5' | -56.6 | NC_001806.1 | + | 127536 | 0.68 | 0.773991 |
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Target: 5'- ---cGGGGUCCuCGGcGGGCUCGGgGu -3' miRNA: 3'- uuuaCCUCAGGuGCCaUCCGGGUCgU- -5' |
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| 5577 | 5' | -56.6 | NC_001806.1 | + | 132941 | 0.66 | 0.868426 |
|
Target: 5'- ---cGGAcccCCACGGccggacGGGCCUAGCAa -3' miRNA: 3'- uuuaCCUca-GGUGCCa-----UCCGGGUCGU- -5' |
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| 5577 | 5' | -56.6 | NC_001806.1 | + | 138840 | 0.72 | 0.500642 |
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Target: 5'- --cUGGGG-CCGCGGgacggGGGCCCGGaCGa -3' miRNA: 3'- uuuACCUCaGGUGCCa----UCCGGGUC-GU- -5' |
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| 5577 | 5' | -56.6 | NC_001806.1 | + | 149424 | 0.68 | 0.72516 |
|
Target: 5'- ---gGGGGUcCCACGGcgaccccGGGCCCuGCGu -3' miRNA: 3'- uuuaCCUCA-GGUGCCa------UCCGGGuCGU- -5' |
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| 5577 | 5' | -56.6 | NC_001806.1 | + | 151110 | 0.67 | 0.792679 |
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Target: 5'- ---gGGGGU-CGCGGgGGGCuCCGGCGc -3' miRNA: 3'- uuuaCCUCAgGUGCCaUCCG-GGUCGU- -5' |
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| 5577 | 5' | -56.6 | NC_001806.1 | + | 151295 | 0.7 | 0.632859 |
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Target: 5'- ---gGGGGgcggCgGCGGUGGGCCgGGCc -3' miRNA: 3'- uuuaCCUCa---GgUGCCAUCCGGgUCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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